Our protocol can be used to produce high quality exosome-enriched extracellular vesicles from embryonic stem cells that express the immune stimulatory factor, GM-CSF. Exosome enriched extracellular vesicles carrying GM-CSF, have the potential to s
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This study describes a method to isolate exosome-enriched extracellular vesicles carrying immune-stimulatory granulocyte macrophage colony-stimulating factors from embryonic stem cells.