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Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

6.6K Views

08:34 min

January 8th, 2020

DOI :

10.3791/60297-v

January 8th, 2020


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Pseudomonas Aeruginosa

Chapters in this video

0:05

Title

1:24

Detection of Double-crossover Recombinants of P. aeruginosa

2:24

Confirmation of Gene Deletion with Colony PCR

3:07

Visualization of Bioluminescence-labeled Bacteria in Mice

5:42

Inoculation of Animals with Bacterial Strains

6:28

Results: Evaluation of Attenuated P. aeruginosa Strain

7:24

Conclusion

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