A Tuberculosis Molecular Bacterial Load Assay (TB-MBLA)
13.8K Views
•
10:41 min
•
April 30th, 2020
DOI :
April 30th, 2020
•Wilber Sabiiti1, Bariki Mtafya1,2, Daniela Alferes De Lima1, Evelin Dombay1, Vincent O. Baron1, Khalide Azam3, Katarina Oravcova4, Derek J. Sloan1, Stephen H. Gillespie1
1Division of Infection and Global Health, School of Medicine, University of St Andrews, 2National Institute for Medical Research (NIMR)-Mbeya Medical Research Centre, 3Instituto Nacional de Saúde (INS), Ministério da Saúde, 4Institute of Biodiversity, Animal Health & Comparative Medicine, College of Medical, Veterinary & Life Sciences, University of Glasgow
Chapters in this video
0:05
Introduction
1:33
Sample Preparation and Heat Inactivation
2:30
RNA Extraction
6:28
Reverse Transcriptase qPCR
7:57
Results: Heat Inactivation of M. tuberculosis Bacilli
9:43
Conclusion
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