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In Vivo Mouse Model of Spinal Implant Infection Video (Video) | JoVE

DOI :

10.3791/60560-v

June 23rd, 2020

June 23rd, 2020

2,012 Views

1Department of Orthopaedic Surgery, University of California Los Angeles, 2David Geffen School of Medicine, University of California Los Angeles, 3University of South Carolina School of Medicine, University of South Carolina

The protocol describes a novel in vivo mouse model of spinal implant infection where a stainless-steel k-wire implant is infected with bioluminescent Staphylococcus aureus Xen36. Bacterial burden is monitored longitudinally with bioluminescent imaging and confirmed with colony forming unit counts after euthanasia.

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