A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

8.3K Views

07:50 min

March 30th, 2020

DOI :

10.3791/60806-v

March 30th, 2020


Transcript

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Multispectral Fluorescence Imaging

Chapters in this video

0:05

Introduction

0:39

Antibody and Fluorophore Selecting and Staining

1:52

Spectral Library Preparation

2:59

Multispectral Imaging

3:54

Spectral Unmixing

4:37

Analyzing Multispectral Images via Cell Segmentation and Phenotyping

5:52

Results: Representative Multispectral Imaging and Cell Quantification

7:07

Conclusion

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