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Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches

14.2K Views

10:05 min

May 23rd, 2020

DOI :

10.3791/60822-v

May 23rd, 2020


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Neurosphere Assay

Chapters in this video

0:04

Introduction

0:59

Postnatal Day 1-3 (P1-3) Mouse Brain Harvest

2:28

Subventricular Zone (SVZ)/Dentate Gyrus (DG) Microdissections

4:22

Tissue Dissociation

5:27

Postnatal Neural Stem Cell Expansion

6:09

Neurosphere Passaging

7:10

Results: Representative Neurosphere Expansion and Characterization

9:21

Conclusion

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