Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions
NC Spheroid Maintenance and Sympathetic Progenitor Induction (Day 11-14)
6:09
Sympathetic Neuron (symN) Differentiation and Maturation (Day 14-20 or 28-35)
7:38
Results: Representative NC Induction, Maintenance, and Expansion
9:43
Conclusion
Transcript
This protocol allows the derivation of sympathetic neurons from human pluripotent stem cells. These cells will enable a scientist to study human disorders that affect the sympathetic nervous system. This technique allows the derivation of sympathe
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In this protocol, we describe a stable, highly efficient differentiation strategy for the generation of postganglionic sympathetic neurons from human pluripotent stem cells. This model will make neurons available for the use of studies of multiple autonomic disorders.