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Surface Passivation for Single-molecule Protein Studies

DOI :

10.3791/50549-v

10:35 min

April 24th, 2014

April 24th, 2014

40,389 Views

1Kavli Institute of NanoScience, Department of BioNanoScience, Delft University of Technology, 2Department of Chemistry, Seoul National University

We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.

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Surface Passivation

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