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Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays

DOI :

10.3791/64881-v

February 24th, 2023

February 24th, 2023

1,290 Views

1Washington University in St. Louis, 2University of California San Francisco

The present protocol describes methods for evaluating DNA damage repair proteins in patient-derived ovarian cancer organoids. Included here are comprehensive plating and staining methods, as well as detailed, objective quantification procedures.

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