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Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional (x, y, z, and λ) Hyperspectral FRET Imaging and Analysis

3.8K Views

08:22 min

October 27th, 2020

DOI :

10.3791/61720-v

October 27th, 2020


Transcript

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3D CAMP Distribution

Chapters in this video

0:04

Introduction

0:34

Imaging Preparation

1:16

Image Acquisition

4:00

Image Analysis

5:22

FRET Efficiency Calculation and Mapping

6:26

Results: Representative 3D cAMP Distribution and Hyperspectral FRET Imaging and Analysis

7:57

Conclusion

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