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Cardiac Spheroids as in vitro Bioengineered Heart Tissues to Study Human Heart Pathophysiology

DOI :

10.3791/61962-v

10:41 min

January 23rd, 2021

January 23rd, 2021

6,817 Views

1University of Newcastle, 2University of Sydney, 3Kolling Institute of Medical Research, Royal North Shore Hospital, 4University of Technology, Sydney

This protocol aims to fabricate 3D cardiac spheroids (CSs) by co-culturing cells in hanging drops. Collagen-embedded CSs are treated with doxorubicin (DOX, a cardiotoxic agent) at physiological concentrations to model heart failure. In vitro testing using DOX-treated CSs may be used to identify novel therapies for heart failure patients.

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Cardiac Spheroids

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