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Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

DOI :

10.3791/62114-v

June 4th, 2021

June 4th, 2021

4,362 Views

1Department of Biochemistry and Molecular Biology, Saitama University, 2Department of Botany, University of Wisconsin

Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.

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