Microscope Setting and Conducting Real-time Imaging
2:48
Data Analysis
4:23
Results: Ca2+ Transmission and Elevation of Apoplastic Glutamate in Response to Mechanical Wounding
6:09
Conclusion
필기록
This protocol allows plant-wide real time imaging of the activity of the plant systemic signaling system through monitoring the dynamics of the calcium and apoplastic glutamate in response to wounding. This plant-wide real-time imaging method prov
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Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.