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Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

4.6K Views

06:50 min

June 4th, 2021

June 4th, 2021

4,630 Views

0:04

Introduction

0:56

Microscope Setting and Conducting Real-time Imaging

2:48

Data Analysis

4:23

Results: Ca2+ Transmission and Elevation of Apoplastic Glutamate in Response to Mechanical Wounding

6:09

Conclusion

Transcribir

This protocol allows plant-wide real time imaging of the activity of the plant systemic signaling system through monitoring the dynamics of the calcium and apoplastic glutamate in response to wounding. This plant-wide real-time imaging method prov

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Extracellular glutamate-triggered systemic calcium signaling is critical for the induction of plant defense responses to mechanical wounding and herbivore attack in plants. This article describes a method to visualize the spatial and temporal dynamics of both these factors using Arabidopsis thaliana plants expressing calcium- and glutamate-sensitive fluorescent biosensors.

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