Results: Morphological and Functional Validation of RPE Cells
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Conclusion
Transcription
This protocol allows for the efficient isolation and culture of mouse RPE cells. Healthy primary mouse RPE cultures are valuable models to understand the mechanisms of underlying eye diseases. Confluent and viable RPE cultures obtain within three
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This protocol, which was originally reported by Fernandez-Godino et al. in 20161, describes a method to efficiently isolate and culture mouse RPE cells, which form a functional and polarized RPE monolayer within one week on Transwell plates. The procedure takes approximately 3 hours.