April 5th, 2021
•Here, a protocol is provided for time-lapse imaging of ocular morphogenesis using a commercially available lightsheet microscope and an image processing workstation to analyze the resulting data. This protocol details the procedures for embryo anesthesia, embedding in low melting temperature agarose, suspension in the imaging chamber, setting up the imaging parameters, and finally analyzing the imaging data using image analysis software.
Related Videos
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
Tracking Cells in GFP-transgenic Zebrafish Using the Photoconvertible PSmOrange System
Analysis of Zebrafish Kidney Development with Time-lapse Imaging Using a Dissecting Microscope Equipped for Optical Sectioning
Using Light Sheet Fluorescence Microscopy to Image Zebrafish Eye Development
Visualizing the Interrenal Steroidogenic Tissue and Its Vascular Microenvironment in Zebrafish
Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy
Generation of Genetically Modified Mice through the Microinjection of Oocytes
High-resolution Episcopic Microscopy (HREM) - Simple and Robust Protocols for Processing and Visualizing Organic Materials
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved