Gene Knock-in by CRISPR/Cas9 and Cell Sorting in Macrophage and T Cell Lines
10.4K Views
•
11:32 min
•
November 13th, 2021
DOI :
November 13th, 2021
•Chapters in this video
0:05
Introduction
0:32
Design and Plasmid Construction of sgRNAs Targeting Rosa26 Locus
2:28
Design and Construction of Targeting Vector as Homologous Recombination Template
3:46
Electroporation of Macrophage and T Cell Lines
7:19
Cell Sorting to Isolate Putative Knock-in Cells
8:58
Screening and Validation of Positive Knock-in Cells
10:16
Representative Results
10:52
Conclusion
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