JoVE Logo
Faculty Resource Center

Sign In

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

DOI :

10.3791/62396-v

May 27th, 2021

May 27th, 2021

3,513 Views

1Department of Physics, Virginia Tech, 2Center for Soft Matter and Biological Physics, Virginia Tech

This paper describes the protocols for sample preparation, data reduction, and data analysis in neutron spin echo (NSE) studies of lipid membranes. Judicious deuterium labeling of lipids enables access to different membrane dynamics on mesoscopic length and time scales, over which vital biological processes occur.

-- Views

Related Videos

article

Evaluation of Lipid Droplet Size and Fusion in Bovine Hepatic Cells

article

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

article

A Precise and Quantifiable Method for Collecting Hemolymph from Small Arthropods

article

Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

article

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells

article

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

article

Imaging ATG9A, a Multi-Spanning Membrane Protein

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

Author Spotlight: Developing a Tool for Using Inverted Confocal Microscopes for In Vivo Intravital Imaging

article

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved