July 29th, 2021
•We present protocols for three different methods for the homogenization of four different muscle groups of rat skeletal muscle tissue to measure and compare the levels of nitrate and nitrite. Furthermore, we compare different sample weights to investigate whether tissue sample size affects the results of homogenization.
Tags
Related Videos
Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells
Procedures for Rat in situ Skeletal Muscle Contractile Properties
Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle
Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle
Tissue Triage and Freezing for Models of Skeletal Muscle Disease
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
Identification, Isolation, and Characterization of Fibro-Adipogenic Progenitors (FAPs) and Myogenic Progenitors (MPs) in Skeletal Muscle in the Rat
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved