Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
3.3K Views
•
15:01 min
•
September 18th, 2021
DOI :
September 18th, 2021
•Ivan V. Maslov1, Andrey O. Bogorodskiy1, Mariia V. Pavelchenko1, Ilia O. Zykov1, Natalya I. Troyanova2, Valentin I. Borshchevskiy1,3, Marina A. Shevchenko2
1Research Center for Molecular Mechanisms of Aging and Age-Related Diseases, Moscow Institute of Physics and Technology, 2Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 3Institute of Biological Information Processing (IBI-7: Structural Biochemistry), Forschungszentrum Jülich
Chapters in this video
0:04
Introduction
1:03
Aspergillus fumigatus Conidia Application & Specimen Preparation
1:22
Mouse Lung Lobe Optical Clearing
2:23
Mouse Lung Lobe Imaging with CLSM
6:50
Image Processing: Surface Rendering
9:23
Image Processing: Mask Correction
12:54
Conidia Quantitative Analysis
13:41
Representative Results
14:07
Conclusions
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