Profiling of H3K4me3 Modification in Plants using Cleavage under Targets and Tagmentation
4.0K Views
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09:48 min
•
April 22nd, 2022
DOI :
April 22nd, 2022
•1College of Agriculture and Biotechnology, Zhejiang University, 2Central Laboratory, State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Zhejiang Academy of Agricultural Sciences, 3State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Hybrid R & D Engineering Center (the Ministry of Education), College of Agriculture, Nanjing Agricultural University, 4Hainan Institute of Zhejiang University
Chapters in this video
0:06
Introduction
1:51
Nuclei Isolation
4:22
Antibody Incubation
6:15
Transposase Incubation
7:24
Tagmentation
7:52
DNA Extraction and NGS Library Construction
8:19
Representative Results
9:25
Conclusion
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