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Production of Adeno-Associated Virus Vectors in Cell Stacks for Preclinical Studies in Large Animal Models

5.8K Views

07:21 min

June 30th, 2021

DOI :

10.3791/62727-v

June 30th, 2021


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Adeno Associated Virus

Chapters in this video

0:04

Introduction

0:46

Double Plasmid Transfection of HEK293 Cells in Cell Stacks

2:30

Harvesting Adeno-Associated Virus AAV and Chemical Lysis of The Transfected HEK293 Cells

3:23

AAV Vector Purification Using Heparin Affinity Chromatography

5:45

Results: Serum Human Monoclonal Antibody (hIgG) Expression in Animals Upon Administration of AAV6.2FF-hIgG

6:41

Conclusion

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