Double Plasmid Transfection of HEK293 Cells in Cell Stacks
2:30
Harvesting Adeno-Associated Virus AAV and Chemical Lysis of The Transfected HEK293 Cells
3:23
AAV Vector Purification Using Heparin Affinity Chromatography
5:45
Results: Serum Human Monoclonal Antibody (hIgG) Expression in Animals Upon Administration of AAV6.2FF-hIgG
6:41
Conclusion
Transcription
This protocol for production of AAV viral vectors is cost-effective and yields high purity, high titer, research-grade AAV for use in preclinical, large animal models. This technique uses adherent HEK293 cells in cell culture chambers, which is ti
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Here we provide a detailed procedure for large-scale production of research-grade AAV vectors using adherent HEK 293 cells grown in cell stacks and affinity chromatography purification. This protocol consistently yields >1 x 1013 vector genomes/mL, providing vector quantities appropriate for large animal studies.