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Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay

2.7K Views

06:51 min

July 21st, 2021

DOI :

10.3791/62762-v

July 21st, 2021


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Chaperone cochaperone Interactions

Chapters in this video

0:04

Introduction

1:28

Coupling of Hsp90 C-Terminal Peptide to the Acceptor Beads

2:49

Assay for Interaction Between GST-FKBP51 or GST-FKBP52 and Hsp90 C-Terminal Peptide and Inhibition with Small Molecular Mass Compounds

4:14

Dose-Response Curve Generation and IC50 Calculation

4:58

Results: Inhibitory Effect of Small Molecular Weight Test Compound D10

5:59

Conclusion

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