Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay
2.7K Views
•
06:51 min
•
July 21st, 2021
DOI :
July 21st, 2021
•Chapters in this video
0:04
Introduction
1:28
Coupling of Hsp90 C-Terminal Peptide to the Acceptor Beads
2:49
Assay for Interaction Between GST-FKBP51 or GST-FKBP52 and Hsp90 C-Terminal Peptide and Inhibition with Small Molecular Mass Compounds
4:14
Dose-Response Curve Generation and IC50 Calculation
4:58
Results: Inhibitory Effect of Small Molecular Weight Test Compound D10
5:59
Conclusion
Related Videos
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
8.7K Views
Quantification of Bacterial Histidine Kinase Autophosphorylation Using a Nitrocellulose Binding Assay
8.5K Views
Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange Mass Spectrometry for Studying Protein Structure and Dynamics
9.7K Views
Using Three-color Single-molecule FRET to Study the Correlation of Protein Interactions
10.0K Views
Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
12.5K Views
A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
11.2K Views
Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
13.5K Views
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
6.5K Views
Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils
5.9K Views
Sample Preparation and Relative Quantitation using Reductive Methylation of Amines for Peptidomics Studies
2.2K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved