JoVE Logo

Sign In

Real-Time Fluorescent Measurement of Synaptic Functions in Models of Amyotrophic Lateral Sclerosis

2.5K Views

08:59 min

July 16th, 2021

DOI :

10.3791/62813-v

July 16th, 2021


Explore More Videos

Real Time Fluorescent Measurement

Chapters in this video

0:04

Introduction

0:52

Microscope and Perfusion System Setup

2:33

Styryl Dye Imaging of Synaptic Vesicle Release

4:30

Fluorescence Imaging of Gcamp6m Calcium Transients

5:36

Image Analysis

6:23

Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons

8:15

Conclusion

Related Videos

article

12:22

Automated Quantification of Synaptic Fluorescence in C. elegans

10.2K Views

article

09:31

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

7.0K Views

article

09:10

DetectSyn: A Rapid, Unbiased Fluorescent Method to Detect Changes in Synapse Density

3.2K Views

article

12:18

The Neuromuscular Junction: Measuring Synapse Size, Fragmentation and Changes in Synaptic Protein Density Using Confocal Fluorescence Microscopy

21.6K Views

article

10:46

Using Enzyme-based Biosensors to Measure Tonic and Phasic Glutamate in Alzheimer's Mouse Models

11.2K Views

article

08:53

Assay to Measure Nucleocytoplasmic Transport in Real Time within Motor Neuron-like NSC-34 Cells

8.6K Views

article

08:27

Single Synapse Indicators of Glutamate Release and Uptake in Acute Brain Slices from Normal and Huntington Mice

6.1K Views

article

07:07

Measuring Glucose Uptake in Drosophila Models of TDP-43 Proteinopathy

2.7K Views

article

09:24

Expanding the Toolkit for In Vivo Imaging of Axonal Transport

3.3K Views

article

09:36

Primary Cultures of Rat Astrocytes and Microglia and Their Use in the Study of Amyotrophic Lateral Sclerosis

3.3K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved