Fluorescence Imaging of Gcamp6m Calcium Transients
5:36
Image Analysis
6:23
Results: Real-Time Monitoring of the Dynamics of Presynaptic Calcium Influx and Synaptic Vesicle Membrane Fusion in In Vitro Cultured Neurons
8:15
Conclusion
Transcription
These methods enable quick assessment as to whether an experimental manipulation, disease causative protein, or RNA can impact synaptic processes, and whether therapeutic compounds can restore function. These techniques provide a reliable readout
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Two related methods are described to visualize subcellular events required for synaptic transmission. These protocols enable the real-time monitoring of the dynamics of presynaptic calcium influx and synaptic vesicle membrane fusion using live-cell imaging of in vitro cultured neurons.