September 20th, 2021
•Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent neurons.
Related Videos
Double Fluorescence in situ Hybridization in Fresh Brain Sections (Video) | JoVE
Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology (Video) | JoVE
Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE) (Video) | JoVE
Detection of the Genome and Transcripts of a Persistent DNA Virus in Neuronal Tissues by Fluorescent In situ Hybridization Combined with Immunostaining Video (Video) | JoVE
Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization Video (Video) | JoVE
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections Video (Video) | JoVE
In Vitro Recording of Mesenteric Afferent Nerve Activity in Mouse Jejunal and Colonic Segments Video (Video) | JoVE
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization Video (Video) | JoVE
Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology Video (Video) | JoVE
Preparations and Protocols for Whole Cell Patch Clamp Recording of Xenopus laevis Tectal Neurons Video (Video) | JoVE
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved