Results I: Visualization of Trypanosoma cruzi‐Infected Cells, Dormant Amastigotes, and Inflammation Foci in Cleared Organs
6:27
Results II: Immunodetection of Vasculature in Cleared Trypanosoma cruzi‐Infected Organs
7:07
Conclusion
Transcript
This technique provided a more complete and 3D image of entire transparent organs and was useful for quantification, not only of replicating, but also dormant parasite and immune factor cells. This technique allows us to determine the special asso
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The present protocol describes light-sheet fluorescent microscopy and automated software-assisted methods to visualize and precisely quantify proliferating and dormant Trypanosoma cruzi parasites and T cells in intact, cleared organs and tissues. These techniques provide a reliable way to evaluate treatment outcomes and offer new insights into parasite-host interactions.