Results: Post-Replicative ssDNA Gaps on Ongoing Forks by S1 Fiber Assay and Post-Replicative Repair by Gap Filling
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Conclusion
Transcript
Our protocol opens new possibilities to investigate how replication occurs on damaged DNA. The techniques can detect the formation and repair of single-stranded DNA gaps, opposite the DNA lesion. The S1 nuclease cleaves the single-stranded DNA and
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Here we describe two modifications of the DNA fiber assay to investigate single-stranded DNA gaps in replicating DNA after lesion induction. The S1 fiber assay enables the detection of post-replicative gaps using the ssDNA-specific S1 endonuclease, while the gap-filling assay allows visualization and quantification of gap repair.