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Imaging Neutrophil Migration in the Mouse Skin to Investigate Subcellular Membrane Remodeling Under Physiological Conditions

1.2K Views

08:41 min

May 10th, 2022

May 10th, 2022

1,227 Views

0:00

Introduction

0:41

Neutrophil Labeling

1:43

Neutrophil Injection

2:55

Imaging

6:14

Results: Study of Immune Cell Recruitment and Function in Tissues

8:14

Conclusion

Transcript

Neutrophil migration relies on the rapid and continuous remodeling of the plasma membrane in response to chemoattractant and its interactions with the extracellular microenvironment. Described herein is a procedure based on Intravital Subcellular Microscopy to investigate the dynamic of membrane remodeling in neutrophils injected in the ear of anesthetized mice.

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