This research was supported by the Purdue University Department of Chemistry startup fund.

1. Instrumental setup for hyperspectral CRS imaging
NOTE: The generation of CRS signal requires the use of high-power (i.e., class 3B or class 4) lasers. Safety protocols must be addressed and proper personal protective equipment (PPE) must be worn at all times when working at such high peak powers. Consult proper documentation before experimentation. This protocol focuses on designing the beam path, chirping the femtosecond pulses, and optimizing imaging conditions. A general optical layout of this hyperspectral CRS microscope is shown in Figure 1. The configuration shown here is one of many existing configurations for CRS microscopy. The CRS microscopy system used in this protocol is built upon a dual-output femtosecond laser source and a laser scanning microscope.
<ol>
	<li>Ensure that the laser source provides two femtosecond pulse trains (120 fs width) with a repetition rate of 80 MHz, including a fixed wavelength at 1,045 nm used as the Stokes beam, and a tunable wavelength from 680 to 1,300 nm used as the pump beam. Synchronize the output pulses with an optical delay difference. Use a microscope frame to construct the imaging platform.</li>
	<li>Designing the beam path
	<ol>
		<li>To control the laser power at the sample, use a half-wave plate and polarization beam splitter (PBS) combination for each laser beam.</li>
		<li>Install an acousto-optic modulator (AOM) in the Stokes laser beam path. Focus the beam with a 150 mm focal length lens into the AOM and recollimate the 0th order output with a 400 mm focal length lens.</li>
		<li>Use the same lens pair (150 mm and 400 mm focal lengths) to expand the pump beam to match the laser beam size with the Stokes.</li>
		<li>Place the 400 mm focal length lenses in both the pump and Stokes beam paths on separate one-dimensional translation stages for fine-tuning the beam divergence and optimizing the beam size before entering the microscope.</li>
		<li>Direct the pump beam with a right-angle reflecting mirror mounted on a motorized translation stage for optical delay tuning. If the Stokes beam needs optical delay, place these components instead into its beam path.</li>
		<li>Allow both beams to be combined at a dichroic mirror with a cutoff wavelength at ~1,000 nm (between the pump and Stokes wavelengths), so that the Stokes beam will transmit through the dichroic mirror while the pump beam is reflected by the dichroic mirror. Send the collinearly combined laser beams to the microscope.</li>
		<li>To chirp the pump and Stokes beams, place glass rods in their beam paths. See step 1.5 for details.</li>
		<li>To confirm proper alignment and beam size, use iris diaphragms after the dichroic mirror and before the microscope. Specifically, install one at a position close to and the other at a distance from the dichroic mirror to confirm good alignment and beam overlap. Use an IR-viewing card or IR viewer to visualize the beam during alignment.</li>
		<li>Use a fast PD and an oscilloscope to approximately measure the optical delay between the pump and Stokes pulses. Trigger the oscilloscope by sampling the laser pulse train.</li>
		<li>Block the pump beam and sample the Stokes beam. Zoom in on one of the pulses and place a vertical cursor on it to mark its temporal location on the oscilloscope.</li>
		<li>Unblock the pump beam and block the Stokes beam. Translate the delay stage until the sample pump pulses temporally align with the marked position.</li>
	</ol>
	</li>
	<li>The laser scanning microscope
	<ol>
		<li>For an upright microscope configuration, send the combined laser beams through a periscope to climb to an appropriate level before reaching the 2D galvo scanning mirrors.</li>
		<li>Measure the laser beam size before the microscope, and set up the proper lens pair after the galvo mirrors to expand the laser beam to best match the size of the entrance pupil of the objective lens.</li>
		<li>Construct a 4-f system using the two lenses, with the back aperture of the objective lens and the center of the two galvo mirrors being conjugate planes. Alternatively, use two separate 1D galvo mirrors with two 4-f lens systems for laser scanning.</li>
		<li>After the condenser, design a 2 in flip mirror to reflect the laser beams for signal collection. Position a lens with a 2 in diameter in the transmitted beam path to fully collect and focus transmission signals to the detectors.</li>
		<li>Direct the CARS signals to the PMT with a dichroic mirror having a cutoff at 776 nm, and allow the transmitted SRS signals to be detected by the PD. Use a bandpass filter (655/30 nm) before the PMT to reject residual excitation laser pulses. Use a short-pass filter (980 nm short pass) before the PD to block the Stokes beam from entering the detector.</li>
		<li>For CARS signal detection, connect a preamplifier and a current-voltage converter after the PMT and before sending the signal to the data acquisition system. Adjust the PMT voltage to optimize the signal and image contrast.</li>
		<li>Use a function generator to modulate the AOM at 1-10 MHz, and use the same frequency as the reference for the lock-in demodulation. Use a lock-in amplifier to extract SRS signals before data acquisition.</li>
	</ol>
	</li>
	<li>Data acquisition and display
	<ol>
		<li>Perform data acquisition using a digital data acquisition (DAQ) card in conjunction with a terminal block.</li>
		<li>Use the analog outputs from the DAQ to control the galvo mirrors and the analog inputs for signal acquisition.</li>
		<li>Use Lab-written software based on LabVIEW having a simultaneous multichannel display for real-time viewing and saving images (see Supplemental File).</li>
	</ol>
	</li>
	<li>Chirping the femtosecond source and measuring the spectral resolution 
	NOTE: To achieve a good spectral resolution using spectral focusing, glass rods are used to introduce dispersions and chirp laser pulses from femtosecond to picosecond. To achieve the best spectral resolution, the chirp rate of the pump beam needs to equal that of the Stokes beam. For this laser system, the best spectral resolution can be achieved by chirping the ~120 fs output laser pulses to 3.4 ps for the pump and 1.8 ps for the Stokes. This chirping is achieved by using a 4+1 combination (four in the combined beam, one only in the Stokes beam) of 150 mm glass rod (SF-57) combination, as described below, and should achieve a 15 cm-1 spectral resolution. The pulse duration can be measured using an autocorrelator.
	<ol>
		<li>Insert one 150 mm glass rod into only the Stokes beam path.</li>
		<li>Insert two 150 mm glass rods into the combined pump/Stokes beam path after the dichroic beam splitter. To increase the chirping, let the combined laser beams double-pass the two glass rods by placing a dielectric mirror at one end of the rods.</li>
		<li>To measure the spectral resolution, prepare a standard chemical (e.g., dimethyl sulfoxide [DMSO]) sample pressed between two glass coverslips, and scan the delay stage until the maximum signal is achieved.</li>
		<li>Move the optical delay 1,000 &#181;m in the red-shift direction. Then, run 200 frames at 10 &#181;m/step toward the blueshift direction to collect the hyperspectral image stack.</li>
		<li>To convert the frame numbers to wavenumbers, perform a linear regression using the symmetric (2,913 cm-1) and asymmetric (2,994 cm-1) C-H stretching from DMSO and their corresponding frame numbers40.</li>
		<li>Use the XPM signal for measuring the spectral focusing intensity profile. Half-close the diaphragm on the condenser and move the focus to a blank coverslip. Collect the same number of steps as for hyperspectral SRS. To measure the CARS nonresonant background, focus on the glass coverslip and collect the same number of steps for the hyperspectral CARS measurements.</li>
	</ol>
	</li>
	<li>Optimizing the signal-to-noise ratio (SNR) of images
	<ol>
		<li>Prepare a chemical sample for system alignment. Follow the procedure described in step 3.1 for sample preparation. 
		NOTE: DMSO is a good choice because it is a common lab chemical with strong Raman signals and well-separated C-H symmetric and asymmetric peaks.</li>
		<li>Place the sample on the microscope stage, and add water or immersion oil if needed for the objective lens or condenser. Properly move the edge of the DMSO droplet in the field of view and adjust the objective lens for the best focus. Center the condenser using the K&#246;hler illumination method41. Fully open the diaphragm on the condenser.</li>
		<li>Tune the pump beam wavelength to 800 nm (1,045 nm Stokes) to target the 2,913 cm-1 CH3 peak. Set the power of both the pump and Stokes beam to ~30 mW before the microscope by adjusting the half-wave plate (~10 mW power at the sample plane).</li>
		<li>For SRS, set the lock-in amplifier gain to ~10 with a time constant of 7 &#181;s (when using a 10 &#181;s pixel dwell time). Ensure that the time constant is smaller than the pixel dwell time. Use Demod R for the AUX output for SRS signals.</li>
		<li>For CARS, send the PMT output to the preamplifier and current-voltage converter. Use the DAQ to acquire the output from the converter.</li>
		<li>Set the image acquisition parameters in the acquisition software. Use a pixel number of 200 x 200 with a scanning size of ~100 x 100 &#181;m2. Make sure the image contains both the DMSO droplet and an empty area.</li>
		<li>Scan the sample and check the image on the computer screen. Scan the motorized delay stage in the Stokes/pump beam while monitoring the real-time images. Scan over the delay until the signal is maximized.</li>
		<li>Move the DMSO droplet to cover the whole field of view and check if the DC signal maximum is centered in the image (signal is pump beam-dependent). Adjust either the position of the pump beam via a mirror or the voltage offset in the imaging software.</li>
		<li>After DC optimization, adjust the Stokes beam mirrors until the AC signal is maximized by adjusting the threshold value to display ~50% saturation. Check whether the saturation is centered in the image. If not, fine-tune the mirrors only in the Stokes beam. Monitor the signal during alignment as real-time feedback on the quality of the alignment.</li>
		<li>To determine the SNR, select a small region of the DMSO image and measure the mean value. For the noise, select a small area in the empty region of the image and determine both the average mean value and the standard deviation. Subtract the noise mean value from the signal mean value and divide the results by the standard deviation of the empty region.</li>
		<li>If the calculated SNR is not high enough (typically 800-1,000 for SRS and &#62;10,000 for CARS at a PMT voltage of 0.4 V with this power combination), check and reoptimize the beam overlap, beam sizes, and delay stage position, fine-tune the AOM, and change the function generator modulation frequency until the expected SNR is obtained.</li>
	</ol>
	</li>
</ol>
2. Image analysis and data processing
<ol>
	<li>SNR analysis
	<ol>
		<li>Open the ImageJ software. To import the saved DMSO sample .txt file, click File | Import | Text Image | Open.</li>
		<li>Once the image is imported, press CTRL+shift+C to bring up the brightness and contrast function (B&#38;C). To find the maximum sample signal, press the auto button in the B&#38;C until a region of the DMSO sample appears saturated.</li>
		<li>Click the oval selection tool on the ImageJ interface and highlight a small area of the saturated DMSO region. Once highlighted, press M to measure the mean and standard deviation of the selected area.</li>
		<li>For measuring the background, adjust the bars in the B&#38;C function until the signal of the empty region can be observed. Click the oval selection and highlight a region of the background the same size as for step 2.1.3. Make sure the region selected does not contain DMSO. Press M to measure the statistics of the selected area.</li>
		<li>Calculate the SNR according to step 1.6.10.</li>
	</ol>
	</li>
	<li>Processing hyperspectral CRS images
	<ol>
		<li>Import the .txt file according to step 2.1.1. Once imported, click on Image | Stacks | Tools | Montage to Stack... to convert the file into an image stack.</li>
		<li>Scroll through the montage until the first DMSO peak is visible. Select a region on the DMSO and click on Image | Stack | Plot Z-axis Profile to plot the intensity versus frame number spectrum. To extract the raw spectral data, click on list and copy the profile data.</li>
		<li>To convert the recovered spectrum into wavenumber units, perform a linear regression as outlined in step 1.5.5.</li>
	</ol>
	</li>
	<li>Fitting for measuring spectral resolution 
	NOTE: Lorentzian functions are used to fit the SRS and CARS spectra28.
	<ol>
		<li>Open the fitting software, then copy and paste the linear regression data into the program. For fitting the SRS data, highlight the data and then plot the data as a scatter plot.</li>
		<li>Pull up the scatter plot. Click on Analysis | Peaks and Baseline | Multiple Peak Fit | Open Dialog to bring up the peak analyzer. When pulled up, check that the input is the current plot and change the peak function to Lorentzian (Lorentz).</li>
		<li>Double-click on each of the two DMSO peaks on the graph to highlight the regions to be fitted. Next, click on Open NLfit to bring up the fitting window. Click on the Fit until converged button, and then OK, to see a tabulated summary of the fitting coefficients (see Eq (3)). 
		NOTE: The below equation shows the Lorentzian function format in the software. A1/2 are the amplitudes of the fitting peaks, w1/2 are the widths of the fitted peaks, and the x01/02 values are the centers of the fitted peaks. The independent variable is x and the dependent variable is y. 
		<img alt="Equation 1" src="/files/ftp_upload/63677/63677eq3v2.png" style="margin: auto;" /> &#160; &#160; (3)</li>
		<li>For CARS spectral fitting, click on Analysis | Fitting | Nonlinear curve fit | Open Dialog. Select category: new to define a new function for CARS. Use a two-peak CARS fitting function defined below (see Eq (4)) for CARS spectral fitting. 
		<img alt="Equation 1" src="/files/ftp_upload/63677/63677eq4v2.png" style="margin: auto;" /> (4)</li>
	</ol>
	</li>
	<li>Determining the spatial resolution 
	NOTE: Before this step, it is important to know the conversion between the pixel size at a specific magnification, pixel number, and the step size in &#181;m. This can be performed by using a sample of a known diameter that is larger than the expected imaging resolution, measuring its line profile, and fitting a Gaussian function to determine the full width at half maximum (FWHM) value. Resolution targets or uniform samples such as polymeric beads can be used.
	<ol>
		<li>Acquire an image of cells or polymer particles less than 200 nm in diameter.</li>
		<li>Use ImageJ to draw a line across the smallest particle in the image.</li>
		<li>Press K to plot the intensity profile.</li>
		<li>Click list from the pop-up window and copy the information to fitting software.</li>
		<li>Plot the profile in fitting software and use Gaussian fitting (click on Analysis | Fitting | Nonlinear curve fit | Open Dialog | Category: Basic Functions; Function: Gauss).</li>
		<li>Read the peak width after fitting. Use the pixel to size conversion to obtain the actual resolution of the microscope.</li>
	</ol>
	</li>
</ol>
3. Preparation of samples for hyperspectral CRS imaging
<ol>
	<li>Preparation of imaging slides and chemical samples
	<ol>
		<li>Place a piece of double-sided tape onto a coverslip, and cut out a small rectangular shape of the tape from the middle of the placed tape to create an open area for the sample to be placed.</li>
		<li>Pipette 1-2 &#181;L of pure DMSO and dispense the droplet at the center of the vacancy.</li>
		<li>Carefully place the top coverslip and gently press the edges of the coverslips to seal the chamber while making sure the DMSO sample does not contact the edges of the tape.</li>
		<li>For sensitivity experiments, prepare serial dilutions of DMSO in deuterium oxide (D2O) to give a concentration range of 50%-0%. Take 1-2 &#181;L of each solution and prepare pressed samples as described above.</li>
	</ol>
	</li>
	<li>Cell preparation
	<ol>
		<li>Seed the cells in a 35 mm glass-bottom dish (or larger) in Dulbecco&#39;s Modified Eagle Medium (DMEM) with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin.</li>
		<li>Incubate the cells in an incubation chamber at 37 &#176;C with a 5% CO2 atmosphere&#160;overnight or longer until ~50%-80% confluency is achieved.</li>
		<li>Image the live cells directly or fix the cells with a 10% formalin solution for imaging.</li>
	</ol>
	</li>
</ol>

The authors declare no conflicts of interest.

The protocol presented here describes the construction of a multimodal CRS microscope and the direct comparison between CARS and SRS imaging. For the microscope construction, the critical steps are spatial and temporal beam overlapping and beam size optimization. It is recommended to use a standard sample such as DMSO before the biological imaging for optimizing SNR and calibrating Raman shifts. Direct comparison between CARS and SRS images reveals that CARS has a better spatial resolution, while SRS gives better spectra...

The Raman scattering phenomenon was first observed in 1928 by C. V. Raman1. When an incident photon is interacting with a sample, an inelastic scattering event can spontaneously occur, in which the energy change of the photon matches a vibrational transition of the analyzed chemical species. This process does not require the use of a chemical tag, making it a versatile, label-free tool for chemical analysis while minimizing sample perturbation. Despite its advantages, spontaneous Raman scattering suffers from a low scattering cross-section (typically 1011 lower than the infrared [IR] absorption cross-section), which necessitates long acquisition times for analysis2. Thus, the quest for increasing the sensitivity of the Raman scattering process is essential in pushing Raman technologies for real-time imaging.
One effective way to greatly enhance the sensitivity of Raman scattering is through coherent Raman scattering (CRS) processes, for which two laser pulses are typically used to excite molecular vibrational transitions3,4. When the photon energy difference between the two lasers matches the vibrational modes of sample molecules, strong Raman signals will be generated. The two most commonly used CRS processes for imaging are coherent anti-Stokes Raman scattering (CARS) and stimulated Raman scattering (SRS)5. Over the past two decades, technological developments have advanced CARS and SRS microscopy techniques to become powerful tools for label-free quantification and elucidation of chemical changes in biological samples.
Chemical imaging by CARS microscopy can be dated to 1982 when laser scanning was first applied to acquire CARS images, demonstrated by Duncan et al6. The modernization of CARS microscopy was greatly accelerated after the wide applications of laser scanning multiphoton fluorescence microscopy7. Early work from the Xie group using high repetition rate lasers has transitioned CARS to be a high-speed, label-free, chemical imaging platform for the characterization of molecules in biological samples8,9,10. One of the major issues for CARS imaging is the presence of a nonresonant background, which reduces the image contrast and distorts the Raman spectrum. Many efforts have been made to either reduce the nonresonant background11,12,13,14,15 or to extract resonant Raman signals from the CARS spectra16,17. Another advancement that has greatly advanced the field is hyperspectral CARS imaging, which allows for spectral mapping at each image pixel with improved chemical selectivity18,19,20,21.
Stimulated Raman scattering (SRS) is a younger imaging technology than CARS, although it was discovered earlier22. In 2007, SRS microscopy was reported using a low repetition rate laser source23. Soon, several groups demonstrated high-speed SRS imaging using high repetition rate lasers24,25,26. One of the major advantages of SRS microscopy over CARS is the absence of the nonresonant background27, although other backgrounds such as cross-phase modulation (XPM), transient absorption (TA), two-photon absorption (TPA), and photothermal (PT) effect, may occur with SRS28. In addition, the SRS signal and sample concentration have linear relationships, unlike CARS, which has a quadratic signal-concentration dependence29. This simplifies chemical quantification and spectral unmixing. Multicolor and hyperspectral SRS has evolved in different forms30,31,32,33,34,35,36, with spectral focusing being one of the most popular approaches for chemical imaging37,38.
Both CARS and SRS require the focusing of the pump and Stokes laser beams onto the sample to match the vibrational transition of the molecules for signal excitation. CARS and SRS microscopes also share a lot in common. However, the physics underlying these two processes, and signal detections involved in these microscopy technologies have disparities3,39. CARS is a parametric process that does not have net photon-molecule energy coupling3. SRS, however, is a nonparametric process, and contributes to energy transfer between photons and molecular systems27. In CARS, a new signal at anti-Stokes frequency is generated, while SRS manifests as the energy transfer between the pump and Stokes laser beams.
CARS signal satisfies Eq (1)28.
<img alt="Equation 1" src="/files/ftp_upload/63677/63677eq1v2.png" style="margin: auto;" /> &#160; &#160; (1)
Meanwhile, SRS signal can be written as Eq (2)28.
<img alt="Equation 1" src="/files/ftp_upload/63677/63677eq2v2.png" style="margin: auto;" />&#160; &#160; (2)
Here, Ip, Is, ICARS, and &#916;ISRS are the intensities of the pump beam, Stokes beam, CARS signal, and SRS signals, respectively.&#160;&#967;(3) is the third-order nonlinear optical susceptibility of the sample, and is a complex value composed of real and imaginary parts.
These equations express the spectral profiles and signal-concentration dependence of CARS and SRS. Differences in physics result in disparate detection schemes for these two microscopy technologies. Signal detection in CARS usually involves spectral separation of newly generated photons and detection using a photomultiplier tube (PMT) or charge-coupled device (CCD); for SRS, the energy exchange between the pump and Stokes beams is usually measured by high-speed intensity modulation using an optical modulator and demodulation using a photodiode (PD) paired with a lock-in amplifier.
Although many technological developments and applications have been published in recent years in both CARS and SRS fields, no systematic comparisons of the two CRS techniques have been performed on the same platform, especially for hyperspectral CARS and SRS microscopy. Direct comparisons in sensitivity, spatial resolution, spectral resolution, and chemical separation capabilities would allow biologists to select the best modality for chemical quantification. In this protocol, detailed steps to construct a multimodal imaging platform with both hyperspectral CARS and SRS modalities based on a femtosecond laser system and spectral focusing are provided. The two techniques have been compared in the forward direction for spectral resolution, detection sensitivity, spatial resolution, and imaging contrasts of cells.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>2D galvo scanner set</td>
			<td>Thorlabs</td>
			<td>GVS002</td>
			<td></td>
		</tr>
		<tr>
			<td>Acousto-optic modulator</td>
			<td>Isomet</td>
			<td>M1205-P80L-0.5</td>
			<td></td>
		</tr>
		<tr>
			<td>AOM driver</td>
			<td>Isomet</td>
			<td>532B-2</td>
			<td></td>
		</tr>
		<tr>
			<td>Data acquisition card</td>
			<td>National Instruments</td>
			<td>PCle 6363</td>
			<td>Custom ordered filter (980 sp)</td>
		</tr>
		<tr>
			<td>Delay stage</td>
			<td>Zaber</td>
			<td>X-LSM050A</td>
			<td></td>
		</tr>
		<tr>
			<td>Deuterium oxide</td>
			<td>Millipore Sigma</td>
			<td>151882-100G</td>
			<td></td>
		</tr>
		<tr>
			<td>Dichroic mirror for beam combination</td>
			<td>Thorlabs</td>
			<td>DMLP1000</td>
			<td></td>
		</tr>
		<tr>
			<td>Dichroic mirror for signal separation</td>
			<td>Semrock</td>
			<td>FF776-Di01-25x36</td>
			<td></td>
		</tr>
		<tr>
			<td>DMSO</td>
			<td>MiliporeSigma</td>
			<td>200-664-3</td>
			<td></td>
		</tr>
		<tr>
			<td>MIA PaCa 2 Cells</td>
			<td>ATCC</td>
			<td>CRL-1420</td>
			<td></td>
		</tr>
		<tr>
			<td>Femtosecond laser system</td>
			<td>Spectral Physics</td>
			<td>InSightX3+</td>
			<td></td>
		</tr>
		<tr>
			<td>Filter for CARS</td>
			<td>Chroma</td>
			<td>AT655/30m</td>
			<td></td>
		</tr>
		<tr>
			<td>Filter for SRS</td>
			<td>Chroma</td>
			<td>ET980sp</td>
			<td></td>
		</tr>
		<tr>
			<td>Function generator</td>
			<td>Rigol</td>
			<td>DG1022Z</td>
			<td></td>
		</tr>
		<tr>
			<td>Glass rods</td>
			<td>Lattice Electro Optics</td>
			<td>SF-57</td>
			<td></td>
		</tr>
		<tr>
			<td>Half-wave plate</td>
			<td>Newport</td>
			<td>10RP02-51; 10RP02-46</td>
			<td></td>
		</tr>
		<tr>
			<td>LabVIEW 2020</td>
			<td>National Instruments</td>
			<td></td>
			<td>This is the image acquisition software</td>
		</tr>
		<tr>
			<td>Lock-in amplifier</td>
			<td>Zurich Instrument</td>
			<td>HF2LI</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscope housing</td>
			<td>Olympus</td>
			<td>BX51W1</td>
			<td></td>
		</tr>
		<tr>
			<td>Objective lens</td>
			<td>Olympus</td>
			<td>UPLSAPO60XW</td>
			<td></td>
		</tr>
		<tr>
			<td>Origin Pro 2019b</td>
			<td>OriginLab Corporation</td>
			<td></td>
			<td>This is the spectral fitting software</td>
		</tr>
		<tr>
			<td>Oscilloscope</td>
			<td>Tektronix</td>
			<td>TBS2204B</td>
			<td></td>
		</tr>
		<tr>
			<td>Photodiode</td>
			<td>Hamamatsu</td>
			<td>S3994-01</td>
			<td></td>
		</tr>
		<tr>
			<td>PMT detector</td>
			<td>Hamamatsu</td>
			<td>H7422P-40</td>
			<td></td>
		</tr>
		<tr>
			<td>PMT voltage amplifier</td>
			<td>Advanced Research Instrument Corp.</td>
			<td>PMT4V3</td>
			<td></td>
		</tr>
		<tr>
			<td>Polarizing beamsplitter cube</td>
			<td>Thorlabs</td>
			<td>PBS255</td>
			<td></td>
		</tr>
		<tr>
			<td>Terminal block</td>
			<td>National Instruments</td>
			<td>BNC-2110</td>
			<td></td>
		</tr>
	</tbody>
</table>

direct comparison of hyperspectral stimulated raman scattering and coherent anti-stokes raman scattering microscopy for chemical imaging

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging technologies. Hyperspectral SRS and CARS imaging offer Raman spectral information at every pixel, which enables better separation of different chemical compositions. Although both techniques require two excitation lasers, their signal detection schemes and spectral properties are quite different. The goal of this protocol is to perform both hyperspectral SRS and CARS imaging on a single platform and compare the two microscopy techniques for imaging different biological samples. The spectral focusing method is employed to acquire spectral information using femtosecond lasers. By using standard chemical samples, the sensitivity, spatial resolution, and spectral resolution of SRS and CARS in the same excitation conditions (i.e., power at the sample, pixel dwell time, objective lens, pulse energy) are compared. The imaging contrasts of CARS and SRS for biological samples are juxtaposed and compared. The direct comparison of CARS and SRS performances would allow for optimal selection of the modality for chemical imaging.

Comparisons of the spectral resolution 
Figure 2 compares the spectral resolution of hyperspectral SRS (Figure 2A) and CARS (Figure 2B) microscopy using a DMSO sample. For the SRS spectrum, two Lorentzian functions (see protocol step 2.3) were applied to fit the spectrum, and a resolution of 14.6 cm-1 was obtained using the 2,913 cm-1 peak. For CARS, a two-peak-fitting function with a Gaus...

Watch this Scientific Journal Video about Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging at JoVE.com

Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity.

Stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) microscopy are the most widely used coherent Raman scattering imaging ...

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JoVE Journal

Chemistry

3.7K Views.  Purdue University. This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity. 

Video: Direct Comparison of Hyperspectral Stimulated Raman Scattering and Coherent Anti-Stokes Raman Scattering Microscopy for Chemical Imaging

A Filter-based Surface Enhanced Raman Spectroscopic Assay for Rapid Detection of Chemical Contaminants

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be applied to the detection of various chemical contaminants. Silver nanoparticles (Ag NPs) are synthesized via reduction of silver nitrate by sodium citrate. Then the NPs are aggregated by sodium chloride to form nanoclusters that could be trapped in the pores of the filter membrane. A syringe is connected to the filter holder, with a filter membrane inside. By loading the nanoclusters into the syringe and passing through the membrane, the liquid goes through the membrane but not the nanoclusters, forming a SERS-active membrane. When testing the analyte, the liquid sample is loaded into the syringe and flowed through the Ag NPs coated membrane. The analyte binds and concentrates on the Ag NPs coated membrane. Then the membrane is detached from the filter holder, air dried and measured by a Raman instrument. Here we present the study of the volume effect of Ag NPs and sample on the detection sensitivity as well as the detection of 10 ppb ferbam and 1 ppm ampicillin using the developed assay.

A procedure for fabrication and performing the filter-based surface enhanced Raman spectroscopic (SERS) assay for the detection of chemical contaminants (i.e., pesticide ferbam and antibiotic ampicillin) is presented.

We demonstrate a method to fabricate highly sensitive surface-enhanced Raman spectroscopic (SERS) substrates using a filter syringe system that can be ...

Preparation of Biopolymer Aerogels Using Green Solvents

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers (gelatin, agar, cellulose), only recently have biomasses been recognized as an abundant source of chemically diverse macromolecules for functional aerogel materials. Biopolymer aerogels (pectin, alginate, chitosan, cellulose, etc.) exhibit both specific inheritable functions of starting biopolymers and distinctive features of aerogels (80-99% porosity and specific surface up to 800 m2/g). This synergy of properties makes biopolymer aerogels promising candidates for a wide gamut of applications such as thermal insulation, tissue engineering and regenerative medicine, drug delivery systems, functional foods, catalysts, adsorbents and sensors. This work demonstrates the use of pressurized carbon dioxide (5 MPa) for the ionic cross linking of amidated pectin into hydrogels. Initially a biopolymer/salt dispersion is prepared in water. Under pressurized CO2 conditions, the pH of the biopolymer solution is lowered to 3 which releases the crosslinking cations from the salt to bind with the biopolymer yielding hydrogels. Solvent exchange to ethanol and further supercritical CO2 drying (10 - 12 MPa) yield aerogels. Obtained aerogels are ultra-porous with low density (as low as 0.02 g/cm3), high specific surface area (350 - 500 m2/g) and pore volume (3 - 7 cm3/g for pore sizes less than 150 nm).

A new way for the production of biopolymer-based aerogels by carbon dioxide (CO2) induced gelation is shown. The technique utilizes pressurized carbon dioxide (5 MPa) for the production of biopolymer hydrogels and supercritical CO2 (12 MPa) to convert gels into aerogels. The only solvents needed besides CO2 are water and ethanol.

Although the first reports on aerogels made by Kistler1 in the 1930s dealt with aerogels from both inorganic oxides (silica and others) and biopolymers ...

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

A protocol for measuring polydispersity of concentrated polymer solutions using dynamic light scattering is described. Dynamic light scattering is a technique used to measure the size distribution of polymer solutions or colloidal particles. Although this technique is widely used for the assessment of polymer solutions, it is difficult to measure the particle size in concentrated solutions due to the multiple scattering effect or strong light absorption. Therefore, the concentrated solutions should be diluted before measurement. Implementation of the confocal optical component in a dynamic light scattering microscope1 helps to overcome this barrier. Using such a microscopic system, both transparent and turbid systems can be analyzed under the same experimental setup without a dilution. As a representative example, a size distribution measurement of a temperature-responsive polymer solution was performed. The sizes of the polymer chains in an aqueous solution were several tens of nanometers at a temperature below the lower critical solution temperature (LCST). In contrast, the sizes increased to more than 1.0 &#181;m when above the LCST. This result is consistent with the observation that the solution turned turbid above the LCST.

A protocol for the direct measurement of particle size distribution in concentrated solutions using dynamic light scattering microscopy is presented.

A protocol for measuring polydispersity of concentrated polymer solutions using dynamic light scattering is described. Dynamic light scattering is a ...

A Novel Technique for Raman Analysis of Highly Radioactive Samples Using Any Standard Micro-Raman Spectrometer

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a tight capsule that isolates the material from the atmosphere. The capsule can optionally be filled with a chosen gas pressurized up to 20 bars. The micro-Raman measurement is performed through an optical-grade quartz window. This technique permits accurate Raman measurements with no need for the spectrometer to be enclosed in an alpha-tight containment. It therefore allows the use of all options of the Raman spectrometer, like multi-wavelength laser excitation, different polarizations, and single or triple spectrometer modes. Some examples of measurements are shown and discussed. First, some spectral features of a highly radioactive americium oxide sample (AmO2) are presented. Then, we report the Raman spectra of neptunium oxide (NpO2) samples, the interpretation of which is greatly improved by employing three different excitation wavelengths, 17O doping, and a triple mode configuration to measure the anti-stokes Raman lines. This last feature also allows the estimation of the sample surface temperature. Finally, data that were measured on a sample from Chernobyl lava, where phases are identified by Raman mapping, are shown.

We present a technique for Raman spectroscopic analysis of highly radioactive samples compatible with any standard micro-Raman spectrometer, without any radioactive contamination of the instrument. We also show some applications using actinide compounds and irradiated fuel materials.

A novel approach for the Raman measurement of nuclear materials is reported in this paper. It consists of the enclosure of the radioactive sample in a ...

Monitoring the Effects of Illumination on the Structure of Conjugated Polymer Gels Using Neutron Scattering

We demonstrate a protocol to effectively monitor the gelation process of a high concentration solution of conjugated polymer both in the presence and absence of white light exposure. By instituting a controlled temperature ramp, the gelation of these materials can be precisely monitored as they proceed through this structural evolution, which effectively mirrors the conditions experienced during the solution deposition phase of organic electronic device fabrication. Using small angle neutron scattering (SANS) and ultra-small angle neutron scattering (USANS) along with appropriate fitting protocols we quantify the evolution of select structural parameters throughout this process. Thorough analysis indicates that continued light exposure throughout the gelation process significantly alters the structure of the ultimately formed gel. Specifically, the aggregation process of poly(3-hexylthiophene-2,5-diyl) (P3HT) nano-scale aggregates is negatively affected by the presence of illumination, ultimately resulting in the retardation of growth in conjugated polymer microstructures and the formation of smaller scale macro-aggregate clusters.

A protocol for the analysis of gels formed from the optoelectronic conjugated polymer poly(3-hexylthiophene-2,5-diyl) (P3HT) using small and ultra-small angle neutron scattering in both the presence and absence of illumination is presented.

We demonstrate a protocol to effectively monitor the gelation process of a high concentration solution of conjugated polymer both in the presence and ...

Observation and Analysis of Blinking Surface-enhanced Raman Scattering

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on how to prepare the SERS-active silver nanoaggregate, record a video of certain blinking spots in the microscopic image, and analyze the blinking statistics. In this analysis, a power law reproduces the probability distributions for bright events relative to their duration. The probability distributions for dark events are fitted by a power law with an exponential function. The parameters of the power law represent molecular behavior in both bright and dark states. The random walk model and the speed of the molecule across the entire silver surface can be estimated. It is difficult to estimate even when using averages, autocorrelation functions, and super-resolution SERS imaging. In the future, power law analyses should be combined with spectral imaging, because the origins of blinking cannot be confirmed by this analysis method alone.

This protocol describes the analysis of blinking surface-enhanced Raman scattering due to the random walk of a single molecule on a silver surface using power laws.

From a single molecule at a silver nanoaggregate junction, blinking surface-enhanced Raman scattering (SERS) is observed. Here, a protocol is presented on ...

Application of Voltage in Dynamic Light Scattering Particle Size Analysis

Dynamic light scattering (DLS) is a common method for characterizing the size distribution of polymers, proteins, and other nano- and microparticles. Modern instrumentation permits measurement of particle size as a function of time and/or temperature, but currently there is no simple method for performing DLS particle size distribution measurements in the presence of applied voltage. The ability to perform such measurements would be useful in the development of electroactive, stimuli-responsive polymers for applications such as sensing, soft robotics, and energy storage. Here, a technique using applied voltage coupled with DLS and a temperature ramp to observe changes in aggregation and particle size in thermoresponsive polymers with and without electroactive monomers is presented. The changes in aggregation behavior observed in these experiments were only possible through the combined application of voltage and temperature control. To obtain these results, a potentiostat was connected to a modified cuvette in order to apply voltage to a solution. Changes in polymer particle size were monitored using DLS in the presence of constant voltage. Simultaneously, current data were produced, which could be compared with particle size data, to understand the relationship between current and particle behavior. The polymer poly(N-isopropylacrylamide) (pNIPAM) served as a test polymer for this technique, as pNIPAM's response to temperature is well-studied. Changes in the lower-critical solution temperature (LCST) aggregation behavior of pNIPAM and poly(N-isopropylacrylamide)-block-poly(ferrocenylmethyl methacrylate), an electrochemically active block-copolymer, in the presence of applied voltage are observed. Understanding the mechanisms behind such changes will be important when trying to achieve reversible polymer structures in the presence of applied voltage.

Here, a protocol to apply voltage to solution during dynamic light scattering particle size measurements with the intent to explore the effect of voltage and temperature changes on polymer aggregation is presented.

Dynamic light scattering (DLS) is a common method for characterizing the size distribution of polymers, proteins, and other nano- and microparticles. ...

Ultrafast Time-resolved Near-IR Stimulated Raman Measurements of Functional &#960;-conjugate Systems

Femtosecond time-resolved stimulated Raman spectroscopy is a promising method of observing the structural dynamics of short-lived transients with near infrared (near-IR) transitions, because it can overcome the low sensitivity of spontaneous Raman spectrometers in the near-IR region. Here, we describe technical details of a femtosecond time-resolved near-IR multiplex stimulated Raman spectrometer that we have recently developed. A description of signal generation and optimization, measurement, data acquisition, and calibration and correction of recorded data is provided as well. We present an application of our spectrometer to analyze the excited-state dynamics of &#946;-carotene in toluene solution. A C=C stretch band of &#946;-carotene in the second lowest excited singlet (S2) state and the lowest excited singlet (S1) state is clearly observed in the recorded time-resolved stimulated Raman spectra. The femtosecond time-resolved near-IR stimulated Raman spectrometer is applicable to the structural dynamics of &#960;-conjugate systems from simple molecules to complex materials.

Details of signal generation and optimization, measurement, data acquisition, and data handling for a femtosecond time-resolved near-IR stimulated Raman spectrometer are described. A near infrared stimulated Raman study on the excited-state dynamics of &#946;-carotene in toluene is shown as a representative application.

Femtosecond time-resolved stimulated Raman spectroscopy is a promising method of observing the structural dynamics of short-lived transients with near ...

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures

Neutron crystallography is a structural technique that allows determination of hydrogen atom positions within biological macromolecules, yielding mechanistically important information about protonation and hydration states while not inducing radiation damage. X-ray diffraction, in contrast, provides only limited information on the position of light atoms and the X-ray beam&#160;rapidly induces radiation damage of photosensitive&#160;cofactors and metal centers. Presented here is the workflow employed for the IMAGINE and MaNDi beamlines at Oak Ridge National Laboratory (ORNL) to obtain a neutron diffraction structure once a protein crystal of suitable size (&#62; 0.1 mm3) has been grown. We demonstrate mounting of hydrogenated protein&#160;crystals in quartz capillaries for neutron diffraction data collection. Also presented is the vapor exchange process of the mounted crystals with D2O-containing buffer to ensure replacement of hydrogen atoms at exchangeable sites with deuterium. The incorporation of deuterium reduces the background arising from the incoherent scattering of hydrogen atoms and prevents density cancellation caused by their negative coherent scattering length. Sample alignment and room temperature data collection strategies are illustrated using quasi-Laue data collection at IMAGINE at the High Flux Isotope Reactor (HFIR). Furthermore, crystal mounting and rapid freezing in liquid nitrogen for cryo-data collection to trap labile reaction intermediates is demonstrated at the MaNDi time-of-flight instrument at the Spallation Neutron Source (SNS). Preparation of the model coordinate and diffraction data files and visualization of the neutron scattering length density (SLD) maps will also be addressed. Structure refinement against neutron data-only or against joint X-ray/neutron data to obtain an all-atom structure of the protein of interest will finally be discussed. The process of determining a neutron structure will be demonstrated using crystals of the lytic polysaccharide monooxygenase Neurospora crassa LPMO9D, a copper-containing metalloprotein involved in the degradation of recalcitrant polysaccharides via oxidative cleavage of the glycosidic bond.

Neutron protein crystallography is a structural technique that permits the localization of hydrogen atoms, thereby providing important mechanistic details of protein function. We present here the workflow for mounting a protein crystal, neutron diffraction data collection, structure refinement and analysis of the neutron scattering length density maps.

Neutron crystallography is a structural technique that allows determination of hydrogen atom positions within biological macromolecules, yielding ...

﻿Analyzing the Heterogeneous Performance of Single Nanoparticles with Electrochemical Methods

Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy

Studying electrochemical reactions on single nanoparticles is important to understand the heterogeneous performance of individual nanoparticles. This nanoscale heterogeneity remains hidden during the ensemble-averaged characterization of nanoparticles. Electrochemical techniques have been developed to measure currents from single nanoparticles but do not provide information about the structure and identity of the molecules that undergo reactions at the electrode surface. Optical techniques such as surface-enhanced Raman scattering (SERS) microscopy and spectroscopy can detect electrochemical events on individual nanoparticles while simultaneously providing information on the vibrational modes of electrode surface species. In this paper, a protocol to track the electrochemical oxidation-reduction of Nile Blue (NB) on single Ag nanoparticles using SERS microscopy and spectroscopy is demonstrated. First, a detailed protocol for fabricating Ag nanoparticles on a smooth and semi-transparent Ag film is described. A dipolar plasmon mode aligned along the optical axis is formed between a single Ag nanoparticle and Ag film. The SERS emission from NB fixed between the nanoparticle and the film is coupled into the plasmon mode, and the high-angle emission is collected by a microscope objective to form a donut-shaped emission pattern. These donut-shaped SERS emission patterns allow for the unambiguous identification of single nanoparticles on the substrate, from which the SERS spectra can be collected. In this work, a method for employing the SERS substrate as a working electrode in an electrochemical cell compatible with an inverted optical microscope is provided. Finally, tracking the electrochemical oxidation-reduction of NB molecules on an individual Ag nanoparticle is shown. The setup and the protocol described here can be modified to study various electrochemical reactions on individual nanoparticles.

Author Spotlight: Tracking Electrochemistry on Single Nanoparticles with Surface-Enhanced Raman Scattering Spectroscopy and Microscopy  

The protocol describes how to monitor electrochemical events on single nanoparticles using surface-enhanced Raman scattering spectroscopy and imaging.

Studying electrochemical reactions on single nanoparticles is important to understand the heterogeneous performance of individual nanoparticles. This ...