Preparation of Samples for Hyperspectral CRS Imaging
1:55
Instrumental Setup for Hyperspectral CRS Imaging
4:18
Image Analysis and Data Processing
6:51
Results: Comparing the Resolution, Sensitivity, and Imaging Contrasts of SRS and CARS
8:54
Conclusion
Transcript
This protocol compares the two most widely-used hyperspectral coherent Raman scattering techniques including the coherent anti-Stokes Raman scattering and the stimulated Raman scattering processes and this allows the biologists to choose the best
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This paper directly compares the resolution, sensitivity, and imaging contrasts of stimulated Raman scattering (SRS) and coherent anti-Stokes Raman scattering (CARS) integrated into the same microscope platform. The results show that CARS has a better spatial resolution, SRS gives better contrasts and spectral resolution, and both methods have similar sensitivity.