Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology
2.3K Views
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06:54 min
•
July 5th, 2022
DOI :
July 5th, 2022
•Miao Huang1,5, Heyang Wang1,5, Alfredo A. Delgado1, Tyler A. Reid1, Julian Long2, Shu Wang3,5, Hayley Sussman4, Juan Guan5,6,7, Hitomi Yamaguchi1, Xin Tang1,5,8,9
1Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, 2Department of Materials Science and Engineering, University of Florida, 3Department of Biostatistics, University of Florida, 4Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), 5UF Health Cancer Center, University of Florida, 6Department of Physics, College of Liberal Arts and Sciences, University of Florida, 7Department of Anatomy and Cell Biology, College of Medicine, University of Florida, 8J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 9Department of Physiology and Functional Genomics, University of Florida
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Chapters in this video
0:04
Introduction
0:36
Cell Culture with Magnetic Microbeads
1:29
Force Application and Live-Cell Imaging
4:34
Results: Application of Mechanical Force on Cell Nucleus and Simultaneous Live-Cell Fluorescent Imaging
6:28
Conclusion
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