Combining 3D Magnetic Force Actuator and Multi-Functional Fluorescence Imaging to Study Nucleus Mechanobiology

2.2K Views

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06:54 min

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July 5th, 2022

DOI :

10.3791/64098-v

July 5th, 2022

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Miao Huang¹^,⁵, Heyang Wang¹^,⁵, Alfredo A. Delgado¹, Tyler A. Reid¹, Julian Long², Shu Wang³^,⁵, Hayley Sussman⁴, Juan Guan⁵^,⁶^,⁷, Hitomi Yamaguchi¹, Xin Tang¹^,⁵^,⁸^,⁹

¹Department of Mechanical and Aerospace Engineering, Herbert Wertheim College of Engineering, University of Florida, ²Department of Materials Science and Engineering, University of Florida, ³Department of Biostatistics, University of Florida, ⁴Department of Biomedical Engineering, College of Engineering (COE), University of Delaware (UD), ⁵UF Health Cancer Center, University of Florida, ⁶Department of Physics, College of Liberal Arts and Sciences, University of Florida, ⁷Department of Anatomy and Cell Biology, College of Medicine, University of Florida, ⁸J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, ⁹Department of Physiology and Functional Genomics, University of Florida

Summary

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3D Magnetic Force Actuator

Multi Functional Fluorescence Imaging

Nucleus Mechanobiology

Force Transmission

Non invasive Method

Magnetic Microbeads

RPMI 1640 Culture Medium

Confocal Fluorescence Imaging

Internalization Timing

Live cell Imaging

YAP Nucleus Configuration

Nuclear Stain Intensity

Three dimensional Imaging

Cell Boundary Visualization

Brightfield Microscopy

Chapters in this video

0:04

Introduction

0:36

Cell Culture with Magnetic Microbeads

1:29

Force Application and Live-Cell Imaging

4:34

Results: Application of Mechanical Force on Cell Nucleus and Simultaneous Live-Cell Fluorescent Imaging

6:28

Conclusion

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