Small Extracellular Vesicle Isolation from hUC‐MSCs
2:48
Characterization of hUC‐MSC‐sEVs
7:14
Results: Nanoparticle Tracking Analysis, Biomarker Expression, and Morphology of Single hUC‐MSC‐sEVs
8:39
Conclusion
Transcript
This is a simple and time effective protocol to isolate and concentrate small extracellular vesicles from mesenchymal stem cells. This technique is easy to be adopted in most labs as it is a benchtop scale and only requires simple instruments for
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This protocol demonstrates how to isolate human umbilical cord-derived mesenchymal stem cells' small extracellular vesicles (hUC-MSC-sEVs) with a simple lab-scale benchtop setting. The size distribution, protein concentration, sEVs markers, and morphology of isolated hUC-MSC-sEVs are characterized by nanoparticle tracking analysis, BCA protein assay, western blot, and transmission electron microscope, respectively.