In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
1.3K Views
•
10:44 min
•
May 5th, 2023
DOI :
May 5th, 2023
•Chapters in this video
0:05
Introduction
1:34
Engineering a Fluorescence-Based Reporter Cell Line
3:10
Designing gRNAs for CRISPR/dCas9-Based Epigenetic Silencing of Target Gene
4:13
Arrayed Transient Delivery of CRISPR/dCas9-Based ETRs in Reporter Cell Line
7:19
Analyzing Transcriptional Activity of Target Gene Over Time
8:11
Results: dCas9 ETR-Mediated Epigenetic Silencing of Human B2M Gene by gRNAs
9:23
Conclusion
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