Detection of Polyfunctional T Cells in Children Vaccinated with Japanese Encephalitis Vaccine via the Flow Cytometry Technique
1.8K Views
•
09:37 min
•
September 23rd, 2022
DOI :
September 23rd, 2022
•Linlin Zhang*1,2, Meng Zhang*1,2,3, Mengjia Liu1,2, Junhong Ai1,2, Jiao Tian1,2, Huizheng Ge4, Ran Wang1,2, Zhengde Xie1,2
1Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, 2Research Unit of Critical Infection in Children, 2019RU016, Chinese Academy of Medical Sciences, 3Department of Pediatric Rehabilitation, Beijing Boai Hospital, School of Rehabilitation Medicine, Capital Medical University, China Rehabilitation Research Center, 4Center of Excellence (Beijing), Becton Dickinson Medical Devices (Shanghai) Co Ltd
Chapters in this video
0:05
Introduction
0:39
Stimulation of PBMCs by Inactivated JEV Particles to Induce Cytokine Expression and Ex Vivo Intracellular Staining
4:28
Flow Cytometry Set-Up, Gating Strategy, and Data Analysis
6:56
Results: Flow Cytometry Analysis of Polyfunctional T Cell Profiles in PBMCs Within Japanese Encephalitis Virus Vaccinated Children
9:12
Conclusion
Related Videos
07:31
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
17.4K Views
10:19
High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
16.2K Views
11:07
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
13.2K Views
12:17
IP-FCM: Immunoprecipitation Detected by Flow Cytometry
17.4K Views
09:04
Measurement of T Cell Alloreactivity Using Imaging Flow Cytometry
13.4K Views
07:06
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
8.7K Views
09:01
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
7.0K Views
09:17
A DNA/Ki67-Based Flow Cytometry Assay for Cell Cycle Analysis of Antigen-Specific CD8 T Cells in Vaccinated Mice
7.0K Views
08:44
Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis
2.3K Views
06:22
Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children
909 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved