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Long-Term Culture and Monitoring of Isolated Caenorhabditis elegans on Solid Media in Multi-Well Devices

1.5K Views

09:32 min

December 9th, 2022

DOI :

10.3791/64681-v

December 9th, 2022


Transcript

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Caenorhabditis Elegans

Chapters in this video

0:05

Introduction

1:03

Preparation of the Multi‐Well Device

3:00

Preparation of the Multi‐Well Device for Media Loading

3:29

Filling the Wells with Low Melting Nematode Growth Media (lmNGM)

5:53

Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals

6:55

Spotting the Wells with Concentrated Bacteria

7:29

Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use

8:00

Results: Correlation of Measured Phenotypes Across Populations in Individual Animals

8:55

Conclusion

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