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Functional Characterization and Visualization of Esophageal Fibroblasts Using Organoid Co-Cultures

DOI :

10.3791/64905-v

11:58 min

January 6th, 2023

January 6th, 2023

1,711 Views

1Department of Cell and Molecular Biology, Karolinska Institutet

Organoid-fibroblast co-cultures provide a model to study the in vivo stem cell niche. Here, a protocol for esophageal organoid-fibroblast co-cultures is described. Additionally, whole mount imaging is used to visualize the fibroblast-organoid interaction.

Tags

Functional Characterization

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