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Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

3.2K Views

10:43 min

December 23rd, 2022

DOI :

10.3791/64676-v

December 23rd, 2022


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Esophageal Squamous Cell Carcinoma

Chapters in this video

0:04

Introduction

1:28

Animal Dissection and Organ Collection

4:39

Isolation of Keratinocytes from the Dissected Mouse Tissue

6:07

Seeding of the Initial Cell Suspension

6:54

Passaging and Cryopreservation of Murine Esophageal Organoids (MEOs)

7:52

Preparation of Organoids for Paraffin Embedding

8:48

Results: Characterization of the Organoids

10:03

Conclusion

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