JoVE Logo
Faculty Resource Center

Sign In

Imaging Plasma Membrane Deformations With pTIRFM

DOI :

10.3791/51334-v

12:28 min

April 2nd, 2014

April 2nd, 2014

13,136 Views

1Department of Biological Sciences, Wayne State University

Polarization-based Total Internal Reflection Fluorescence Microscopy (pTIRFM) enables real-time detection of cell membrane dynamics. This article describes the implementation of pTIRFM for the study of membrane remodeling during regulated exocytosis. The technique is generalizable to other processes in cell biology that directly or indirectly involve changes in membrane shape.

Tags

Plasma Membrane Deformations

-- Views

Related Videos

article

Measuring Near Plasma Membrane and Global Intracellular Calcium Dynamics in Astrocytes

article

Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

article

Quantitative Measurement of GLUT4 Translocation to the Plasma Membrane by Flow Cytometry

article

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

article

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

article

Real-time Analyses of Retinol Transport by the Membrane Receptor of Plasma Retinol Binding Protein

article

Ground State Depletion Super-resolution Imaging in Mammalian Cells

article

Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

article

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

article

Isolating and Imaging Live, Intact Pacemaker Regions of Mouse Renal Pelvis by Vibratome Sectioning

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved