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Imaging Plasma Membrane Deformations With pTIRFM

DOI :

10.3791/51334-v

12:28 min

April 2nd, 2014

April 2nd, 2014

13,128 Views

1Department of Biological Sciences, Wayne State University

Polarization-based Total Internal Reflection Fluorescence Microscopy (pTIRFM) enables real-time detection of cell membrane dynamics. This article describes the implementation of pTIRFM for the study of membrane remodeling during regulated exocytosis. The technique is generalizable to other processes in cell biology that directly or indirectly involve changes in membrane shape.

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Plasma Membrane Deformations

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