نشكر الدكتور مايكل وتن لمساعدته في إعداد المخطوط. وأيد هذا العمل من قبل المعاهد الوطنية للصحة 2RO1NS033661 (MWW).

<ol>
	<li>Banker, G. A., Cowan, W. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rat+hippocampal+neurons+in+dispersed+cell+culture.">Rat hippocampal neurons in dispersed cell culture.</a> Brain Res. 126, 397-442 (1977).</li><li>Brewer, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serum-free+B27/neurobasal+medium+supports+differentiated+growth+of+neurons+from+the+striatum,+substantia+nigra,+septum,+cerebral+cortex,+cerebellum,+and+dentate+gyrus.">Serum-free B27/neurobasal medium supports differentiated growth of neurons from the striatum, substantia nigra, septum, cerebral cortex, cerebellum, and dentate gyrus.</a> J. Neurosci. Res. 42, 674-683 (1995).</li><li>Brewer, G. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isolation+and+culture+of+adult+rat+hippocampal+neurons.">Isolation and culture of adult rat hippocampal neurons.</a> J. Neurosci. Methods. 71, 143-155 (1997).</li><li>Brewer, G. J., Torricelli, J. R., Evege, E. K., Price, P. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optimized+survival+of+hippocampal+neurons+in+B27-supplemented+Neurobasal,+a+new+serum-free+medium+combination.">Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination.</a> J. Neurosci. Res. 35, 567-576 (1993).</li><li>Burwell, R. D., Saddoris, M. P., Bucci, D. J., Wiig, K. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Corticohippocampal+contributions+to+spatial+and+contextual+learning.">Corticohippocampal contributions to spatial and contextual learning.</a> J. Neurosci. 24, 3826-3836 (2004).</li><li>Gluck, M. A., Myers, C., Meeter, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cortico-hippocampal+interaction+and+adaptive+stimulus+representation:+a+neurocomputational+theory+of+associative+learning+and+memory.">Cortico-hippocampal interaction and adaptive stimulus representation: a neurocomputational theory of associative learning and memory.</a> Neural Netw. 18, 1265-1279 (2005).</li><li>Kaech, S., Banker, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Culturing+hippocampal+neurons.">Culturing hippocampal neurons.</a> Nat. Protoc. 1, 2406-2415 (2006).</li><li>Mao, L., Wang, J. Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gliogenesis+in+the+striatum+of+the+adult+rat:+alteration+in+neural+progenitor+population+after+psychostimulant+exposure.">Gliogenesis in the striatum of the adult rat: alteration in neural progenitor population after psychostimulant exposure.</a> Brain Res. Dev. Brain Res. 130, 41-51 (2001).</li><li>Mao, L., Wang, J. Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Upregulation+of+preprodynorphin+and+preproenkephalin+mRNA+expression+by+selective+activation+of+group+I+metabotropic+glutamate+receptors+in+characterized+primary+cultures+of+rat+striatal+neurons.">Upregulation of preprodynorphin and preproenkephalin mRNA expression by selective activation of group I metabotropic glutamate receptors in characterized primary cultures of rat striatal neurons.</a> Brain Res. Mol. Brain Res. 86, 125-137 (2001).</li><li>Oorschot, D. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+fluorodeoxyuridine+on+neurons+and+non-neuronal+cells+in+cerebral+explants.">Effect of fluorodeoxyuridine on neurons and non-neuronal cells in cerebral explants.</a> Exp. Brain Res. 78, 132-138 (1989).</li><li>Price, P. J., Brewer, G. J., Federoff, S., Richardson, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Serum+-free+media+for+neural+cell+cultures.">Serum -free media for neural cell cultures.</a> Protocols for Neural Cell Culture. , (2001).</li><li>Shen, J., Watanabe, S., Kaneko, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cell+dissociation+with+papain+reduces+the+density+of+cGMP-activated+channels+of+the+retinal+rod.">Cell dissociation with papain reduces the density of cGMP-activated channels of the retinal rod.</a> Jpn. J. Physiol. 45, 151-164 (1995).</li><li>Stratmann, G., Sall, J. W., May, L. D., Loepke, A. W., Lee, M. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beyond+anesthetic+properties:+The+effects+of+isoflurane+on+brain+cell+death,+neurogenesis+and+long-term+neurocognitive+function.">Beyond anesthetic properties: The effects of isoflurane on brain cell death, neurogenesis and long-term neurocognitive function.</a> Anesthesia and Analgesia. 110, 431-437 (2009).</li><li>Wallace, T. L., Johnson, E. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cytosine+arabinoside+kills+postmitotic+neurons:+evidence+that+deoxycytidine+may+have+a+role+in+neuronal+survival+that+is+independent+of+DNA+synthesis.">Cytosine arabinoside kills postmitotic neurons: evidence that deoxycytidine may have a role in neuronal survival that is independent of DNA synthesis.</a> J. Neurosci. 9, 115-124 (1989).</li></ol>

الإعلان عن أي تضارب في المصالح.

وقد استخدمت الثقافات قرن آمون في البيولوجيا الجزيئية لأكثر من 20 عاما. في حين من حيث المبدأ، ويمكن إجراء الثقافات الخلايا العصبية من أي جزء من الدماغ، وقد أثبتت الثقافات الحصين ليكون الأكثر شعبية نظرا لبنية بسيطة نسبيا من سكان الخلية العصبية في قرن آمون 7. تكون ع...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><td> اسم الكاشف </td><td> بائع </td><td> فهرس العدد </td></tr><tr><td> جرذ الكولاجين الذيل 1 </td><td> العلوم البيولوجية دينار بحريني </td><td> 354236 </td></tr><tr><td> بولي-D-يسين الحل </td><td> Chemicon </td><td> A-003-E </td></tr><tr><td> هانكس المتوازن محلول ملحي </td><td> إينفيتروجن </td><td> 14175-095 </td></tr><tr><td> التربسين الحل (1X) 0.25٪، سائلة </td><td> إينفيتروجن </td><td> 15050-065 </td></tr><tr><td> NeuroBasal متوسطة (1X) السائل </td><td> إينفيتروجن </td><td> 21103-049 </td></tr><tr><td> B27 الملحق (50X) السائل </td><td> إينفيتروجن </td><td> 17504-044 </td></tr><tr><td> الجلوتامين L-200 ملم (100X) السائل </td><td> إينفيتروجن </td><td> 25030-149 </td></tr><tr><td> بنسلين (10،000 وحدة / مل) / الستربتوميسين (10000 ميكروغرام / مل) </td><td> إينفيتروجن </td><td> 15140-148 </td></tr><tr><td> مرحبا الجهات المانحة الحصان مصل </td><td> اتلانتا البيولوجية </td><td> S12150H </td></tr></tbody></table>

isolation and culture of hippocampal neurons from prenatal mice

وتستخدم على نطاق واسع الثقافات الأولية من الفئران والخلايا العصبية قرن آمون الفئران للكشف عن الآليات الخلوية في علم الأعصاب. من خلال عزل وتنامي الخلايا العصبية الفردية، والباحثين قادرون على تحليل الخصائص المتعلقة بالاتجار الخلوية، وبنية الخلية والفردية توطين بروتين باستخدام مجموعة متنوعة من التقنيات الحيوية. نتائج من مثل هذه التجارب لاختبار حاسم نظريات معالجة أساس العصبية للذاكرة والتعلم. ومع ذلك، وتستند النتائج لا لبس فيه من هذه الأشكال من التجارب على قدرته على النمو مع الثقافات العصبية تلوث الحد الأدنى من غيرها من أنواع خلايا الدماغ. في هذا البروتوكول، ونحن نستخدم وسائل الاعلام الخاصة المصممة لنمو الخلايا العصبية وتشريح دقيق من الأنسجة الجنينية الحصين لتحسين نمو الخلايا العصبية السليمة مع التقليل من أنواع الخلايا الملوثة (أي الخلايا النجمية). يمكن الجنينية الماوس الأنسجة الحصين يكون من الصعب عزل من الأنسجة القوارض مماثلة نظرا لحجم العينة لديssection. نعرض تقنيات تشريح مفصل من الحصين من الجنينية الجراء الفار يوم 19 (E19). بمجرد أن يتم عزل الأنسجة قرن آمون، ويتحقق انفصال لطيف من الخلايا العصبية مع تركيز مخفف من التربسين وتعطيل آلية تهدف إلى خلايا منفصلة من النسيج الضام مع توفير الحد الأدنى من الضرر لخلايا فردية. ويرد وصف مفصل لكيفية تحضير الماصات لاستخدامها في تعطيل. وتقدم كثافة الطلاء الأمثل لالمناعة مضان بروتوكولات لتعظيم نجاح زراعة الخلايا. ويقدم هذا البروتوكول تقنية سريع (حوالي 2 ساعة) وفعالة للثقافة من الخلايا العصبية من الأنسجة الماوس قرن آمون.

Watch this Scientific Journal Video about Isolation and Culture of Hippocampal Neurons from Prenatal Mice at JoVE.com

Isolation and Culture of Hippocampal Neurons from Prenatal Mice

ونحن نقدم بروتوكول للثقافة عالي النقاء الخلايا العصبية قرن آمون من أدمغة فئران قبل الولادة من دون استخدام خلية طبقة المغذية الغروية.

العزلة والثقافة من الخلايا العصبية قرن آمون من الفئران قبل الولادة

Primary cultures of rat and murine hippocampal neurons are widely used to reveal cellular mechanisms in neurobiology. By isolating and growing individual ...

isolation-and-culture-of-hippocampal-neurons-from-prenatal-mice

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Neuroscience

70.2K Views.  Auburn University. ونحن نقدم بروتوكول للثقافة عالي النقاء الخلايا العصبية قرن آمون من أدمغة فئران قبل الولادة من دون استخدام خلية طبقة المغذية الغروية.

Video: Isolation and Culture of Hippocampal Neurons from Prenatal Mice

Dissection and Culture of Commissural Neurons from Embryonic Spinal Cord

Commissural neurons have been widely used to investigate the mechanisms underlying axon guidance during embryonic spinal cord development. The cell bodies of these neurons are located in the dorsal spinal cord and their axons follow stereotyped trajectories during embryonic development. Commissural axons initially project ventrally towards the floorplate. After crossing the midline, these axons turn anteriorly and project towards the brain. Each of these steps is regulated by the action of several guidance cues. Cultures highly enriched in commissural neurons are ideally suited for many experiments addressing the mechanisms of axon pathfinding, including turning assays, immunochemistry and biochemistry. Here, we describe a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. First, the spinal cord is isolated and dorsal strips are dissected out. The dorsal tissue is then dissociated into a cell suspension by trypsinization and mechanical disruption. Neurons are plated onto poly-L-lysine-coated glass coverslips or tissue-culture dishes. After 30 hours in vitro, most neurons have extended an axon. The purity of the culture (Yam et al. 2009), typically over 90%, can be assessed by immunolabeling with the commissural neuron markers DCC, LH2 and TAG1 (Helms and Johnson, 1998). This neuronal preparation is a useful tool for in vitro studies of the cellular and molecular mechanisms of commissural axon growth and guidance during spinal cord development.

This video demonstrates a method to dissect and culture commissural neurons from E13 rat dorsal spinal cord. Dissociated commissural neurons are useful to study the cellular and molecular mechanisms of axon growth and guidance.

تشريح والثقافة من الخلايا العصبية من النخاع الشوكي صواري الجنينية

Commissural neurons have been widely used to investigate the mechanisms underlying axon guidance during embryonic spinal cord development. The cell bodies ...

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

Hippocampal and cortical neurons have been used extensively to study central nervous system (CNS) neuronal polarization, axon/dendrite outgrowth, and synapse formation and function. An advantage of culturing these neurons is that they readily polarize, forming distinctive axons and dendrites, on a two dimensional substrate at very low densities. This property has made them extremely useful for determining many aspects of neuronal development. Furthermore, by providing glial conditioning for these neurons they will continue to develop, forming functional synaptic connections and surviving for several months in culture. In this protocol we outline a technique to dissect, culture and transfect embryonic mouse hippocampal and cortical neurons. Transfection is accomplished by electroporating DNA into the neurons before plating via nucleofection. This protocol has the advantage of expressing fluorescently-tagged fusion proteins early in development (~4-8hrs after plating) to study the dynamics and function of proteins during polarization, axon outgrowth and branching. We have also discovered that this single transfection before plating maintains fluorescently-tagged fusion protein expression at levels appropriate for imaging throughout the lifetime of the neuron (&gt; 2 months in culture). Thus, this methodology is useful for studying protein localization and function throughout CNS development with little or no disruption of neuronal function.

This protocol outlines the steps required to dissect, transfect via electroporation and culture mouse hippocampal and cortical neurons. Short-term cultures may be used for studies of axon outgrowth and guidance, while long-term cultures can be used for studies of synaptogenesis and dendritic spine analysis.

Nucleofection والثقافة الأولية من الخلايا العصبية الجنينية الماوس الحصين والقشرية

Hippocampal and cortical neurons have been used extensively to study central nervous system (CNS) neuronal polarization, axon/dendrite outgrowth, and ...

Calcium Phosphate Transfection of Primary Hippocampal Neurons

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this method on hard-to-transfect cells like primary neurons. Here we describe our detailed protocol for calcium phosphate transfection of hippocampal neurons cocultured with astroglial cells.

الكالسيوم فوسفات ترنسفكأيشن من الخلايا العصبية الحصين الابتدائية

Calcium phosphate precipitation is a convenient and economical method for transfection of cultured cells. With optimization, it is possible to use this ...

Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

Neurons are multifaceted cells that carry information essential for a variety of functions including sensation, motor movement, learning, and memory. Studying neurons in vivo can be challenging due to their complexity, their varied and dynamic environments, and technical limitations. For these reasons, studying neurons in vitro can prove beneficial to unravel the complex mysteries of neurons. The well-defined nature of cell culture models provides detailed control over environmental conditions and variables. Here we describe how to isolate, dissociate, and culture primary neurons from chick embryos. This technique is rapid, inexpensive, and generates robustly growing sensory neurons. The procedure consistently produces cultures that are highly enriched for neurons and has very few non-neuronal cells (less than 5%). Primary neurons do not adhere well to untreated glass or tissue culture plastic, therefore detailed procedures to create two distinct, well-defined laminin-containing substrata for neuronal plating are described. Cultured neurons are highly amenable to multiple cellular and molecular techniques, including co-immunoprecipitation, live cell imagining, RNAi, and immunocytochemistry. Procedures for double immunocytochemistry on these cultured neurons have been optimized and described here.

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

العزلة وثقافة فصل الخلايا العصبية الحسية من الفرخ الأجنة

Neurons are multifaceted cells that carry information essential for a variety of functions including sensation, motor movement, learning, and memory. ...

Isolation, Culture and Long-Term Maintenance of Primary Mesencephalic Dopaminergic Neurons From Embryonic Rodent Brains

Degeneration of mesencephalic dopaminergic (mesDA) neurons is the pathological hallmark of Parkinson&#8217;s diseae. Study of the biological processes involved in physiological functions and vulnerability and death of these neurons is imparative to understanding the underlying causes and unraveling the cure for this common neurodegenerative disorder. Primary cultures of mesDA neurons provide a tool for investigation of the molecular, biochemical and electrophysiological properties, in order to understand the development, long-term survival and degeneration of these neurons during the course of disease. Here we present a detailed method for the isolation, culturing and maintenance of midbrain dopaminergic neurons from E12.5 mouse (or E14.5 rat) embryos. Optimized cell culture conditions in this protocol result in presence of axonal and dendritic projections, synaptic connections and other neuronal morphological properties, which make the cultures suitable for study of the physiological, cell biological and molecular characteristics of this neuronal population.

The causes of degeneration of midbrain dopaminergic neurons during Parkinson&#8217;s disease are not fully understood. Cellular culture systems provide an essential tool for study of the neurophysiological properties of these neurons. Here we present an optimized protocol, which can be utilized for in vitro modeling of neurodegeneration.

العزلة والثقافة وطويل الأجل صيانة الابتدائية الدماغ المتوسط ​​الدوبامين العصبية من المخ الجنينية القوارض

Degeneration of mesencephalic dopaminergic (mesDA) neurons is the pathological hallmark of Parkinson&#8217;s diseae. Study of the biological processes ...

Low-Density Primary Hippocampal Neuron Culture

The ability to probe the structure and physiology of individual nerve cells in culture is crucial to the study of neurobiology, and allows for flexibility in genetic and chemical manipulation of individual cells or defined networks. Such ease of manipulation is simpler in the reduced culture system when compared to the intact brain tissue. While many methods for the isolation and growth of these primary neurons exist, each has its own limitations. This protocol describes a method for culturing low-density and high-purity rodent embryonic hippocampal neurons on glass coverslips, which are then suspended over a monolayer of glial cells. This &#39;sandwich culture&#39; allows for exclusive long-term growth of a population of neurons while allowing for trophic support from the underlying glial monolayer. When neurons are of sufficient age or maturity level, the neuron coverslips can be flipped-out of the glial dish and used in imaging or functional assays. Neurons grown by this method typically survive for several weeks and develop extensive arbors, synaptic connections, and network properties.

This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.

منخفض الكثافة الابتدائية الحصين العصبية الثقافة

The ability to probe the structure and physiology of individual nerve cells in culture is crucial to the study of neurobiology, and allows for flexibility ...

Spinal Cord Neurons Isolation and Culture from Neonatal Mice

We present a protocol for the isolation and culture of spinal cord neurons. The neurons are obtained from neonatal C57BL/6 mice and are isolated on postnatal day 1-3. A mouse litter, usually 4-10 pups born from one breeding pair, is gathered for one experiment, and spinal cords are collected individually from each mouse after euthanasia with isoflurane. The spinal column is dissected out and then the spinal cord is released from the column. The spinal cords are then minced to increase the surface area of delivery for an enzymatic protease that allows for the neurons and other cells to be released from the tissue. Trituration is then used to release the cells into solution. This solution is subsequently fractionated in a density gradient to separate the various cells in solution, allowing for neurons to be isolated. Approximately 1-2.5 x 106 neurons can be isolated from one litter group. The neurons are then seeded onto wells coated with adhesive factors that allow for proper growth and maturation. The neurons take approximately 7 days to reach maturity in the growth and culture medium and can be used thereafter for treatment and analysis.

This study presents a technique for the isolation of neurons from WT neonatal mice. It requires the careful dissection of the spinal cord from the neonatal mouse, followed by the separation of neurons from the spinal cord tissue through mechanical and enzymatic cleavage.

الحبل الشوكي العصبونات العزلة والثقافة من الفئران حديثي الولادة

We present a protocol for the isolation and culture of spinal cord neurons. The neurons are obtained from neonatal C57BL/6 mice and are isolated on ...

Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum

The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model system is to provide a controlled microenvironment and maintain the high survival rate and the natural features of dissociated neuronal and nonneuronal cells as much as possible under in vitro conditions. In this article, we demonstrate a method of isolating primary neurons from the developing mouse cerebellum, placing them in an in vitro environment, establishing their growth, and monitoring their viability and differentiation for several weeks. This method is applicable to embryonic neurons dissociated from cerebellum between embryonic days 12&ndash;18.

Conducting in vitro experiments to reflect in vivo conditions as adequately as possible is not an easy task. The use of primary cell cultures is an important step toward understanding cell biology in a whole organism. The provided protocol outlines how to successfully grow and culture embryonic mouse cerebellar neurons.

الثقافة الاساسيه للخلايا العصبية معزولة عن المخيخ الفئران الجنينية

The use of primary cell cultures has become one of the major tools to study the nervous system in vitro. The ultimate goal of using this simplified model ...

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice

Oculomotor neurons (CN3s) and trochlear neurons (CN4s) exhibit remarkable resistance to degenerative motor neuron diseases such as amyotrophic lateral sclerosis (ALS) when compared to spinal motor neurons (SMNs). The ability to isolate and culture primary mouse CN3s, CN4s, and SMNs would provide an approach to study mechanisms underlying this selective vulnerability. To date, most protocols use heterogeneous cell cultures, which can confound the interpretation of experimental outcomes. To minimize the problems associated with mixed-cell populations, pure cultures are indispensable. Here, the first protocol describes in detail how to efficiently purify and cultivate CN3s/CN4s alongside SMNs counterparts from the same embryos using embryonic day 11.5 (E11.5) IslMN:GFP transgenic mouse embryos. The protocol provides details on the tissue dissection and dissociation, FACS-based cell isolation, and in vitro cultivation of cells from CN3/CN4 and SMN nuclei. This protocol adds a novel in vitro CN3/CN4 culture system to existing protocols and simultaneously provides a pure species- and age-matched SMN culture for comparison. Analyses focusing on the morphological, cellular, molecular, and electrophysiological characteristics of motor neurons are feasible in this culture system. This protocol will enable research into the mechanisms that define motor neuron development, selective vulnerability, and disease.

Isolation and Culture of Oculomotor, Trochlear, and Spinal Motor Neurons from Prenatal Islmn:GFP Transgenic Mice

This work presents a protocol to yield homogeneous cell cultures of primary oculomotor, trochlear, and spinal motor neurons. These cultures can be used for comparative analyses of the morphological, cellular, molecular, and electrophysiological characteristics of ocular and spinal motor neurons.

العزلة والثقافة من محرك للعين ، تروشلير ، والخلايا العصبية موتور الشوكي من السابق للولادة Islmn: Gfp الفئران المحورة وراثيا

Oculomotor neurons (CN3s) and trochlear neurons (CN4s) exhibit remarkable resistance to degenerative motor neuron diseases such as amyotrophic lateral ...

Isolation and Culture of Primary Neurons and Glia from Adult Rat Urinary Bladder

The lower urinary tract has two main functions, namely, periodic urine storage and micturition; these functions are mediated through central and peripheral neuroregulation. Although extensive research on the lower urinary tract nervous system has been conducted, most studies have focused on primary culture. This protocol introduces a method for the isolation and culture of bladder neurons and glia from Sprague&#8211;Dawley rats. In this method, the neurons and glia were incubated in a 37 &#176;C, 5% CO2 incubator for 5&#8211;7 days. As a result, they grew into mature shapes suitable for related subsequent immunofluorescence experiments. Cells were morphologically observed using an optical microscope. Neurons, synaptic vesicles, and glia were identified by &#946;-III-tubulin and MAP-2, Synapsin-1, and GFAP staining, respectively. Meanwhile, immunocytochemistry was performed on several neurotransmitter-related proteins, such as choline acetyltransferase, DYNLL2, and SLC17A9.

This protocol attempts to establish a repeatable protocol for primary neurons and glia isolation from rat bladder for further cellular experiments.

العزلة والثقافة من الخلايا العصبية قرن آمون من الفئران قبل الولادة

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Chapters in this video

تشريح والثقافة من الخلايا العصبية من النخاع الشوكي صواري الجنينية

Nucleofection والثقافة الأولية من الخلايا العصبية الجنينية الماوس الحصين والقشرية

الكالسيوم فوسفات ترنسفكأيشن من الخلايا العصبية الحصين الابتدائية

العزلة وثقافة فصل الخلايا العصبية الحسية من الفرخ الأجنة

العزلة والثقافة وطويل الأجل صيانة الابتدائية الدماغ المتوسط الدوبامين العصبية من المخ الجنينية القوارض

منخفض الكثافة الابتدائية الحصين العصبية الثقافة

الحبل الشوكي العصبونات العزلة والثقافة من الفئران حديثي الولادة

الثقافة الاساسيه للخلايا العصبية معزولة عن المخيخ الفئران الجنينية

العزلة والثقافة من محرك للعين ، تروشلير ، والخلايا العصبية موتور الشوكي من السابق للولادة Isl^mn: Gfp الفئران المحورة وراثيا

عزل وثقافة الخلايا العصبية الأولية وغليا من المثانة البولية الجرذ الكبار

العزلة والثقافة من الخلايا العصبية قرن آمون من الفئران قبل الولادة

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العزلة والثقافة وطويل الأجل صيانة الابتدائية الدماغ المتوسط ​​الدوبامين العصبية من المخ الجنينية القوارض

منخفض الكثافة الابتدائية الحصين العصبية الثقافة

الحبل الشوكي العصبونات العزلة والثقافة من الفئران حديثي الولادة

الثقافة الاساسيه للخلايا العصبية معزولة عن المخيخ الفئران الجنينية

العزلة والثقافة من محرك للعين ، تروشلير ، والخلايا العصبية موتور الشوكي من السابق للولادة Islmn: Gfp الفئران المحورة وراثيا

عزل وثقافة الخلايا العصبية الأولية وغليا من المثانة البولية الجرذ الكبار

العزلة والثقافة وطويل الأجل صيانة الابتدائية الدماغ المتوسط الدوبامين العصبية من المخ الجنينية القوارض

العزلة والثقافة من محرك للعين ، تروشلير ، والخلايا العصبية موتور الشوكي من السابق للولادة Isl^mn: Gfp الفئران المحورة وراثيا