An Optimized O9-1/Hydrogel System for Studying Mechanical Signals in Neural Crest Cells

2.5K Views

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11:02 min

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August 13th, 2021

DOI :

10.3791/62693-v

August 13th, 2021

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Tram P. Le*¹, Xiaolei Zhao*¹, Shannon Erhardt¹^,², Jianhua Gu³, Huie Wang³, Tina O. Findley¹, Jun Wang¹^,²

¹Department of Pediatrics, McGovern Medical School, The University of Texas Health Science Center at Houston, ²The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, The University of Texas Health Science Center at Houston, ³SEM AFM Core in the Houston Methodist Hospital Research Institute

Summary

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Aminopropyl Triethoxysilane APTS

Glutaraldehyde

Dichloromethane Silane DCMS

Siliconized Cover Slips

Acrylamide Gel Solution

Chapters in this video

0:05

Introduction

0:34

Preparation of Aminosilane-Coated and Siliconized Glass Coverslips

3:13

Preparation of Hydrogels

6:40

Molecular Analysis of Stiffness via Immunofluorescence Staining

8:04

Results: Characterization of O9-1 Cells Grown on Control and Modified Hydrogel System

10:31

Conclusion

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