JoVE Logo
Faculty Resource Center

Sign In

Culturing and Manipulation of O9-1 Neural Crest Cells

DOI :

10.3791/58346-v

October 9th, 2018

October 9th, 2018

9,330 Views

1Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Biochemistry and Molecular Biology, University of Southern California

O9-1 is a multipotent mouse neural crest cell line. Here we describe detailed step-by-step protocols for culturing O9-1 cells, differentiating O9-1 cells into specific cell types, and genetically manipulating O9-1 cells by using siRNA-mediated knockdown or CRISPR-Cas9 genome editing.

-- Views

Related Videos

article

Flow-sorting and Exome Sequencing of the Reed-Sternberg Cells of Classical Hodgkin Lymphoma

article

The Visual Colorimetric Detection of Multi-nucleotide Polymorphisms on a Pneumatic Droplet Manipulation Platform

article

Genetic Manipulation of the Plant Pathogen Ustilago maydis to Study Fungal Biology and Plant Microbe Interactions

article

Immunostaining for DNA Modifications: Computational Analysis of Confocal Images

article

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

article

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

article

Manipulation of Ploidy in Caenorhabditis elegans

article

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

article

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells

article

Manipulation of Gene Function in Mexican Cavefish

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved