Cost-Efficient Transcriptomic-Based Drug Screening
1.2K Views
•
06:40 min
•
February 23rd, 2024
DOI :
February 23rd, 2024
•Jacqueline Leidner1,2, Heidi Theis3, Michael Kraut3, Alice Ragogna1, Marc Beyer1,3,4, Joachim Schultze1,2,3, Jonas Schulte-Schrepping1,2, Caterina Carraro1,2, Lorenzo Bonaguro1,2
1Systems Medicine, German Center for Neurodegenerative Diseases (DZNE) eV., 2Genomics & Immunoregulation, LIMES Institute, University of Bonn, 3PRECISE Platform for Single Cell Genomics and Epigenomics, DZNE and University of Bonn, 4Immunogenomics & Neurodegeneration, German Center for Neurodegenerative Diseases (DZNE) eV.
Chapters in this video
0:00
Introduction
1:08
Drug Screening Using Peripheral Blood Mononuclear Cells (PBMCs) and Cell Lysate Preparation for mRNA Sequencing
3:01
cDNA Library Preparation from the Drug Treated‐Peripheral Blood Mononuclear Cell (PBMC) Lysate for mRNA Sequencing
5:31
mRNA Sequencing to Understand the Cellular Effect of Drug Treatment
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