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In vivo and in vitro Studies of Adaptor-clathrin Interaction

DOI :

10.3791/2352-v

January 26th, 2011

January 26th, 2011

14,330 Views

1Department of Biochemistry and Molecular Biology, Colorado State University

Clathrin-mediated endocytosis depends on adaptor proteins that coordinate cargo selection and clathrin coat assembly. Here we describe procedures to study adaptor-clathrin physical interaction and live cell imaging approaches using as a model the yeast endocytic adaptor protein Sla1p.

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In Vivo

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