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JoVE Journal

Bioengineering

This content is Open Access.

Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound

9.4K Views

08:51 min

May 8th, 2012

May 8th, 2012

9,352 Views

0:05

Title

1:34

Coating Surfaces with Matrix Ligand

2:54

Preparation of Cells

3:50

Preparing the Low-intensity Pulsed Ultrasound (LIPUS) Stimulation

5:10

Focal Adhesion Analysis by Immunofluorescence

5:58

Biochemical Assays

6:24

Representative Results

8:18

Conclusion

This protocol describes the stimulation of cultured fibroblasts with low-intensity pulsed ultrasound, which drives focal adhesion formation and Rac1 activation by mimicking engagement of the transmembrane matrix receptor, syndecan-4. This approach allows investigation of a successful clinical technique at the cellular level, thereby providing opportunities for refinement of the therapy.

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