Results: Infection and Detection of Merkel Cell Polyomavirus in Human Dermal Fibroblasts
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Conclusion
副本
We presented the first cell culture model for Merkel Cell Polyomavirus infection. The protocol includes isolation of the dermal fibroblasts, preparation of the MCPyV virus infection, immunofluorescent staining, and the fluorescent in situ hybridiz
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Here, we present a protocol to infect primary human dermal fibroblast with MCPyV. The protocol includes isolation of dermal fibroblasts, preparation of MCPyV virions, virus infection, immunofluorescence staining, and fluorescence in situ hybridization. This protocol can be extended for characterizing MCPyV-host interactions and discovering other cell types infectable by MCPyV.
