Construction of ADE2 or LUC1 Reporter Yeast Strain that Contains a Specific RE (yAFM-RE or yLFM-RE)
3:06
Evaluation of P53 Protein Transactivation Ability using the Qualitative Color-based ADE2 Yeast Assay
4:27
Evaluation of P53 Protein Transactivation Ability using the Quantitative Luminescence-based LUC1 Yeast Assay
7:06
Evaluation of P53 Protein Growth Inhibition using the Yeast Phenotypic Assay
8:08
Results
13:51
Conclusion
副本
Hello, my name is Bartolomeo Bosco, and I'm a PhD student at the Laboratory of Transcriptional Network at the Centre for Integrative Biology of the University of Trento in Italy. As you know, transcription is an extremely complex process involving
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Presented here are four protocols to construct and exploit yeast Saccharomyces cerevisiae reporter strains to study human P53 transactivation potential, impacts of its various cancer-associated mutations, co-expressed interacting proteins, and the effects of specific small molecules.
