Co-extraction of PLs, eCBs and Dual Extraction of RNA and Lipids
7:51
Results: Lipidomic and Transcription Profiling in KA-induced Epilepsy
9:27
Conclusion
副本
This modular protocol makes it possible to explore multiple molecular events rendered by structural and signaling lipids, and/or RNAs, in discreet tissue regions and in blood with an improved quantitative and qualitative data output per sample spe
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This article presents a modular protocol for tissue lipidomics and transcriptomics, and plasma lipidomics in neurological disease mouse models targeting lipids underlying inflammation and neuronal activity, membrane lipids, downstream messengers, and mRNA-encoding enzymes/receptors underlying lipid function. Sampling, sample processing, extraction, and quantification procedures are outlined.
